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FMOC-Lys(5/6-FAM)-OH
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Application notes
FMOC-Lys(5/6-FAM)-OH is a building block for in-sequence Lys labeling by FAM. FAM is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2).
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(5/6-FAM)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 137.603 µL | 688.013 µL | 1.376 mL | 6.88 mL | 13.76 mL |
| 5 mM | 27.521 µL | 137.603 µL | 275.205 µL | 1.376 mL | 2.752 mL |
| 10 mM | 13.76 µL | 68.801 µL | 137.603 µL | 688.013 µL | 1.376 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| FMOC-Asp(5/6-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
| FMOC-Glu(5/6-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
| FMOC-Lys(5/6-TAMRA)-OH | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 5 citations: Citation Explorer
Single-step purification of a small non-mAb biologic by peptide-ELP-based affinity precipitation
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides
Authors: Fukunaga, Keisuke and others,
Journal: (2014)
Authors: Fukunaga, Keisuke and others,
Journal: (2014)
Folate-targeted proteolytic nanobeacons: towards selective, imaged delivery in solid tumors
Authors: McFadden, Ian David
Journal: (2014)
Authors: McFadden, Ian David
Journal: (2014)
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
References
View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109