FMOC-Lys(5-FAM)-OH CAS 1242933-88-5

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Physical properties

Molecular weight	726.73
Solvent	DMF

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

1242933-88-5

Molecular weight

726.73

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

83000

Excitation (nm)

493

Emission (nm)

517

FMOC-Lys(5-FAM)-OH is a building block for in-sequence Lys labeling by FAM. FAM is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2).

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.603 µL	688.013 µL	1.376 mL	6.88 mL	13.76 mL
5 mM	27.521 µL	137.603 µL	275.205 µL	1.376 mL	2.752 mL
10 mM	13.76 µL	68.801 µL	137.603 µL	688.013 µL	1.376 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
FMOC-Asp(5-FAM)-OH	493	517	83000	0.32	0.178
FMOC-Glu(5-FAM)-OH	493	517	83000	0.32	0.178
FMOC-Lys(5-TAMRA)-OH	552	578	90000	0.32	0.178
FMOC-Lys(5-FITC)-OH CAS 1095493-05-2	491	516	73000	-	0.35

Images

Figure 1. Chemical structure for FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*

Citations

View all 6 citations: Citation Explorer

Single-step purification of a small non-mAb biologic by peptide-ELP-based affinity precipitation
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784

Development of Phage Biopanning Strategies to Identify Affinity Peptide Ligands for Kappa Light Chain Fab Fragments
Authors: Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Biotechnology progress (2019)

Effect of the hydrophilic-hydrophobic balance of antigen-loaded peptide nanofibers on their cellular uptake, cellular toxicity, and immune stimulatory properties
Authors: Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki
Journal: International journal of molecular sciences (2019): 3781

Purification of Proteins using Peptide-ELP Based Affinity Precipitation
Authors: Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Journal of Biotechnology (2019)

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196

A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65

Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689

Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308

Controlled peptide solvation in portion-mixing libraries of FRET peptides: improved specificity determination for Dengue 2 virus NS2B-NS3 protease and human cathepsin S
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627

Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209