FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*
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Physical properties
Molecular weight | 726.73 |
Solvent | DMF |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 83000 |
Excitation (nm) | 493 |
Emission (nm) | 517 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
CAS 1242933-88-5 | Molecular weight 726.73 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 83000 | Excitation (nm) 493 | Emission (nm) 517 |
FMOC-Lys(5-FAM)-OH is a building block for in-sequence Lys labeling by FAM. FAM is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2).
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 137.603 µL | 688.013 µL | 1.376 mL | 6.88 mL | 13.76 mL |
5 mM | 27.521 µL | 137.603 µL | 275.205 µL | 1.376 mL | 2.752 mL |
10 mM | 13.76 µL | 68.801 µL | 137.603 µL | 688.013 µL | 1.376 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 83000 |
Excitation (nm) | 493 |
Emission (nm) | 517 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
FMOC-Asp(5-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
FMOC-Glu(5-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
FMOC-Lys(5-TAMRA)-OH | 552 | 578 | 90000 | 0.32 | 0.178 |
FMOC-Lys(5-FITC)-OH *CAS 1095493-05-2* | 491 | 516 | 73000 | - | 0.35 |
Citations
View all 6 citations: Citation Explorer
Single-step purification of a small non-mAb biologic by peptide-ELP-based affinity precipitation
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Development of Phage Biopanning Strategies to Identify Affinity Peptide Ligands for Kappa Light Chain Fab Fragments
Authors: Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Biotechnology progress (2019)
Authors: Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Biotechnology progress (2019)
Effect of the hydrophilic-hydrophobic balance of antigen-loaded peptide nanofibers on their cellular uptake, cellular toxicity, and immune stimulatory properties
Authors: Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki
Journal: International journal of molecular sciences (2019): 3781
Authors: Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki
Journal: International journal of molecular sciences (2019): 3781
Purification of Proteins using Peptide-ELP Based Affinity Precipitation
Authors: Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Journal of Biotechnology (2019)
Authors: Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Journal of Biotechnology (2019)
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
References
View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689
Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308
Controlled peptide solvation in portion-mixing libraries of FRET peptides: improved specificity determination for Dengue 2 virus NS2B-NS3 protease and human cathepsin S
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627
Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension
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Bio-inspired NADH regeneration by carbon nitride photocatalysis using diatom templates
Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension
Identification of an Annonaceous Acetogenin Mimetic, AA005, as an AMPK Activator and Autophagy Inducer in Colon Cancer Cells