FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*

FMOC-Lys(5-FAM)-OH

CAS 1242933-88-5

FMOC-Lys(5-FAM)-OH is a building block for in-sequence Lys labeling by FAM. FAM is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5043	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.603 µL	688.013 µL	1.376 mL	6.88 mL	13.76 mL
5 mM	27.521 µL	137.603 µL	275.205 µL	1.376 mL	2.752 mL
10 mM	13.76 µL	68.801 µL	137.603 µL	688.013 µL	1.376 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	726.73 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Single-step purification of a small non-mAb biologic by peptide-ELP-based affinity precipitation

Authors:

Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj

Journal:

Biotechnology and Bioengineering (2020): 3775--3784

Effect of the hydrophilic-hydrophobic balance of antigen-loaded peptide nanofibers on their cellular uptake, cellular toxicity, and immune stimulatory properties

Authors:

Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki

Journal:

International journal of molecular sciences (2019): 3781

Development of Phage Biopanning Strategies to Identify Affinity Peptide Ligands for Kappa Light Chain Fab Fragments

Authors:

Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven

Journal:

Biotechnology progress (2019)

Purification of Proteins using Peptide-ELP Based Affinity Precipitation

Authors:

Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven

Journal:

Journal of Biotechnology (2019)

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7

Authors:

Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi

Journal:

Molecules (2014): 2481--2496

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach

Authors:

Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.

Journal:

Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

Authors:

Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.

Journal:

Nucleic Acids Res (2009): e116

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil

Authors:

Oquare BY, Taylor JS.

Journal:

Bioconjug Chem (2008): 2196

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds

Authors:

Nettels D, Hoffmann A, Schuler B.

Journal:

J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection

Authors:

Bruno JG, Carrillo MP, Phillips T.

Journal:

J Biomol Tech (2008): 109

Physical properties

Molecular weight	726.73
Solvent	DMF

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	1242933-88-5

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