FMOC-Lys(QSY-21)-OH

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Physical properties

Molecular weight	1032.23
Solvent	DMF

Spectral properties

Absorbance (nm)	660
Correction Factor (280 nm)	0.32
Extinction coefficient (cm ^-1 M ^-1)	89000¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1032.23

Absorbance (nm)

660

Correction Factor (280 nm)

0.32

Extinction coefficient (cm ^-1 M ^-1)

89000¹

FMOC-Lys(QSY-21)-OH is a building block for in-sequence Lys labeling by QSY-21. QSY-21 is a quencher that can be used to develop a FRET probe with Cy5 or other fluorescent dyes that have the similar fluorescence spectra (to Cy5) such asTF5 or iFluor 647 dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(QSY-21)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.878 µL	484.388 µL	968.776 µL	4.844 mL	9.688 mL
5 mM	19.376 µL	96.878 µL	193.755 µL	968.776 µL	1.938 mL
10 mM	9.688 µL	48.439 µL	96.878 µL	484.388 µL	968.776 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	660
Correction Factor (280 nm)	0.32
Extinction coefficient (cm ^-1 M ^-1)	89000¹

Product Family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
FMOC-Lys(QSY-7)-OH	90000¹	0.22

Images

Figure 1. Chemical structure for FMOC-Lys(QSY-21)-OH.

References

View all 12 references: Citation Explorer

A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide.
Authors: Ji, Ao and Fan, Yichong and Ren, Wei and Zhang, Shen and Ai, Hui-Wang
Journal: ACS sensors (2018): 992-997

Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy.
Authors: Szymanski, Craig J and Yi, Hong and Liu, Joshua L and Wright, Elizabeth R and Payne, Christine K
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 21-33

Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells.
Authors: Takahashi, Shodai and Piao, Wen and Matsumura, Yuriko and Komatsu, Toru and Ueno, Tasuku and Terai, Takuya and Kamachi, Toshiaki and Kohno, Masahiro and Nagano, Tetsuo and Hanaoka, Kenjiro
Journal: Journal of the American Chemical Society (2012): 19588-91

Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice.
Authors: de la Zerda, Adam and Bodapati, Sunil and Teed, Robert and May, Salomón Y and Tabakman, Scott M and Liu, Zhuang and Khuri-Yakub, Butrus T and Chen, Xiaoyuan and Dai, Hongjie and Gambhir, Sanjiv S
Journal: ACS nano (2012): 4694-701

A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics.
Authors: Kabelác, Martin and Zimandl, Filip and Fessl, Tomás and Chval, Zdenek and Lankas, Filip
Journal: Physical chemistry chemical physics : PCCP (2010): 9677-84

Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
Authors: Jablonski, Amy E and Kawakami, Takashi and Ting, Alice Y and Payne, Christine K
Journal: The journal of physical chemistry letters (2010): 1312-1315

Dissolved core alginate microspheres as "smart-tattoo" glucose sensors.
Authors: Chaudhary, Ayesha and Raina, Monica and McShane, Michael J and Srivastava, Rohit
Journal: Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engin (2009): 4098-101

An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging.
Authors: Maxwell, Dustin and Chang, Qing and Zhang, Xu and Barnett, Edward M and Piwnica-Worms, David
Journal: Bioconjugate chemistry (2009): 702-9

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer.
Authors: Ogawa, Mikako and Kosaka, Nobuyuki and Choyke, Peter L and Kobayashi, Hisataka
Journal: Bioconjugate chemistry (2009): 147-54

Evaluation of glucose sensitive affinity binding assay entrapped in fluorescent dissolved-core alginate microspheres.
Authors: Chaudhary, Ayesha and Raina, Monica and Harma, Harri and Hanninen, Pekka and McShane, Michael J and Srivastava, Rohit
Journal: Biotechnology and bioengineering (2009): 1075-85