FMOC-Lys(QSY-7)-OH

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Physical properties

Molecular weight	1008.20
Solvent	DMF

Spectral properties

Absorbance (nm)	561
Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	90000¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1008.20

Absorbance (nm)

561

Correction Factor (280 nm)

0.22

Extinction coefficient (cm ^-1 M ^-1)

90000¹

FMOC-Lys(QSY-7)-OH is a building block for in-sequence Lys labeling by QSY-7. QSY-7 is a quencher that can be used to develop a FRET probe with Cy3, Alexa Fluor 555, iFluor 555, DyLight 555, ATTO 550, ATTO 565 or other fluorescent dyes that have the similar fluorescence spectra (to Cy3) such asTF3 dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(QSY-7)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	99.187 µL	495.933 µL	991.867 µL	4.959 mL	9.919 mL
5 mM	19.837 µL	99.187 µL	198.373 µL	991.867 µL	1.984 mL
10 mM	9.919 µL	49.593 µL	99.187 µL	495.933 µL	991.867 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	561
Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	90000¹

Product Family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
FMOC-Lys(QSY-21)-OH	89000¹	0.32

Images

Figure 1. Chemical structure for FMOC-Lys(QSY-7)-OH.

References

View all 10 references: Citation Explorer

In Situ Protease Secretion Visualization and Metastatic Lymph Nodes Imaging via a Cell Membrane-Anchored Upconversion Nanoprobe.
Authors: Fang, Yanyun and Li, Yuetong and Li, Yuyi and He, Rong and Zhang, Yue and Zhang, Xiaobo and Liu, Ying and Ju, Huangxian
Journal: Analytical chemistry (2021): 7258-7265

Development of a FRET-Based Assay for Analysis of mAbs Internalization and Processing by Dendritic Cells in Preclinical Immunogenicity Risk Assessment.
Authors: Wen, Yi and Cahya, Suntara and Zeng, Wei and Lin, Joanne and Wang, Xiaoli and Liu, Ling and Malherbe, Laurent and Siegel, Robert and Ferrante, Andrea and Kaliyaperumal, Arunan
Journal: The AAPS journal (2020): 68

Activatable Photodynamic Therapy with Therapeutic Effect Prediction Based on a Self-correction Upconversion Nanoprobe.
Authors: Li, Yuyi and Zhang, Xiaobo and Zhang, Yue and Zhang, Yue and He, Yuling and Liu, Ying and Ju, Huangxian
Journal: ACS applied materials & interfaces (2020): 19313-19323

Visible Multiphoton Dissociation of Chromophore-Tagged Peptides.
Authors: Bouakil, Mathilde and Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: Journal of the American Society for Mass Spectrometry (2017): 2181-2188

An Activatable Fluorescent γ-Polyglutamic Acid Complex for Sentinel Lymph Node Imaging.
Authors: Hagimori, Masayori and Hatabe, Eri and Sano, Kohei and Miyazaki, Hirotaka and Sasaki, Hitoshi and Saji, Hideo and Mukai, Takahiro
Journal: Biological & pharmaceutical bulletin (2017): 297-302

The green tea polyphenol (2)-epigallocatechin-3-gallate (EGCG) is not a β-secretase inhibitor.
Authors: Cheng, Xiao-Rui and Zhou, Jin-Wu and Zhou, Yu and Cheng, Jun-Ping and Yang, Ri-Fang and Zhou, Wen-Xia and Zhang, Yong-xiang and Yun, Liu-Hong
Journal: Bioorganic & medicinal chemistry letters (2012): 1408-14

Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET.
Authors: Le Reste, Ludovic and Hohlbein, Johannes and Gryte, Kristofer and Kapanidis, Achillefs N
Journal: Biophysical journal (2012): 2658-68

Evaluating the 3C-like protease activity of SARS-Coronavirus: recommendations for standardized assays for drug discovery.
Authors: Grum-Tokars, Valerie and Ratia, Kiira and Begaye, Adrian and Baker, Susan C and Mesecar, Andrew D
Journal: Virus research (2008): 63-73

Effects of fluorescent dyes, quenchers, and dangling ends on DNA duplex stability.
Authors: Moreira, Bernardo G and You, Yong and Behlke, Mark A and Owczarzy, Richard
Journal: Biochemical and biophysical research communications (2005): 473-84

Fluorophore-quencher based activatable targeted optical probes for detecting in vivo cancer metastases.
Authors: Ogawa, Mikako and Kosaka, Nobuyuki and Longmire, Michelle R and Urano, Yasuteru and Choyke, Peter L and Kobayashi, Hisataka
Journal: Molecular pharmaceutics : 386-95