Fura-FF, pentapotassium salt [Fura-2FF, pentapotassium salt] *CAS 192140-58-2*

Fura-FF, pentapotassium salt

Fura-2FF, pentapotassium salt; CAS 192140-58-2

Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. The cell-impermeant Fura-2FF is an analog of Fura-2 with much lower calcium binding affinity, Kd ~10 µM.

Datasheet

Catalog	Size	Price	Quantity
21028	10x50 ug	Price
21029	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fura-FF, pentapotassium salt [Fura-2FF, pentapotassium salt] *CAS 192140-58-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.104 µL	585.521 µL	1.171 mL	5.855 mL	11.71 mL
5 mM	23.421 µL	117.104 µL	234.208 µL	1.171 mL	2.342 mL
10 mM	11.71 µL	58.552 µL	117.104 µL	585.521 µL	1.171 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	853.94 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux

Authors:

Garbincius, Joanne F and Salik, Oniel and Cohen, Henry M and Choya-Foces, Carmen and Mangold, Adam S and Makhoul, Angelina D and Schmidt, Anna E and Khalil, Dima Y and Doolittle, Joshua J and Wilkinson, Anya S and others,

Journal:

Nature Metabolism (2025): 1--16

FtsZ filament structures in different nucleotide states reveal the mechanism of assembly dynamics

Authors:

Ruiz, Federico M and Huecas, Sonia and Santos-Aledo, Alicia and Prim, Elena A and Andreu, Jos{\'e} M and Fern{\'a}ndez-Tornero, Carlos

Journal:

PLoS biology (2022): e3001497

An essential role of NAD (P) H oxidase 2 in UVA-induced calcium oscillations in mast cells

Authors:

Li, Zhi Ying and Jiang, Wen Yi and Cui, Zong Jie

Journal:

Photochemical & Photobiological Sciences (2015): 414--428

Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst--A novel mechanism for secretory blockade in acute pancreatitis?

Authors:

Liang, Hui Yuan and Song, Zhi Min and Cui, Zong Jie

Journal:

Biochemical and biophysical research communications (2013): 361--367

References

View all 119 references: Citation Explorer

Load of calcium probe Fura -2/AM in Escherichia coli cells

Authors:

Shao M, Wang HM, Liu ZH, Shen P, Cai RX.

Journal:

Wei Sheng Wu Xue Bao (2005): 805

An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell

Authors:

McHugh JM, Kenyon JL.

Journal:

Am J Physiol Cell Physiol (2004): C342

Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2

Authors:

Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.

Journal:

J Fluoresc (2004): 711

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Authors:

McConnell G, Riis E.

Journal:

Phys Med Biol (2004): 4757

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2

Authors:

Xu T, Yang W, Huo XL, Song T.

Journal:

J Biochem Biophys Methods (2004): 219

Physical properties

Dissociation constant (K_d, nM)	5500
Molecular weight	853.94
Solvent	Water

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	192140-58-2

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