Helixyte™ 14 [Equivalent to SYBR 14]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 474.06 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 489 |
| Emission (nm) | 520 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| Helixyte™ 14 [Equivalent to SYBR 14] *5 mM* |
Related products
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus
Molecular weight 474.06 | Excitation (nm) 489 | Emission (nm) 520 |
Helixyte™ 14 has the same chemical structure of SYBR 14 (SYBR is the trademark of ThermoFisher). It is commonly used to monitor the viability of sperms. The proportion of living sperm in semen can be assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). Helixyte™ 14 is a nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. It stains the nuclei of living sperm bright green. Conversely, PI stains only nonmotile sperm that had lost their membrane integrity. The proportions of living and dead sperm can be determined by first staining with SYBR-14 and PI and then assessing stain uptake by flow cytometry. The proportions of living and dead sperm in mammalian semen can be readily identified through use of dual staining with SYBR-14 and PI and quantified through use of flow cytometry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Helixyte™ 14 [Equivalent to SYBR 14] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 210.944 µL | 1.055 mL | 2.109 mL | 10.547 mL | 21.094 mL |
| 5 mM | 42.189 µL | 210.944 µL | 421.888 µL | 2.109 mL | 4.219 mL |
| 10 mM | 21.094 µL | 105.472 µL | 210.944 µL | 1.055 mL | 2.109 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) |
| Helixyte™ Green *20X Aqueous PCR Solution* | 498 | 522 |
| Helixyte™ Green *10,000X Aqueous PCR Solution* | 498 | 522 |