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Helixyte™ 14 [Equivalent to SYBR 14]

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight474.06
SolventDMSO
Spectral properties
Excitation (nm)489
Emission (nm)520
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
474.06
Excitation (nm)
489
Emission (nm)
520
Helixyte™ 14 has the same chemical structure of SYBR 14 (SYBR is the trademark of ThermoFisher). It is commonly used to monitor the viability of sperms. The proportion of living sperm in semen can be assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). Helixyte™ 14 is a nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. It stains the nuclei of living sperm bright green. Conversely, PI stains only nonmotile sperm that had lost their membrane integrity. The proportions of living and dead sperm can be determined by first staining with SYBR-14 and PI and then assessing stain uptake by flow cytometry. The proportions of living and dead sperm in mammalian semen can be readily identified through use of dual staining with SYBR-14 and PI and quantified through use of flow cytometry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Helixyte™ 14 [Equivalent to SYBR 14] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM210.944 µL1.055 mL2.109 mL10.547 mL21.094 mL
5 mM42.189 µL210.944 µL421.888 µL2.109 mL4.219 mL
10 mM21.094 µL105.472 µL210.944 µL1.055 mL2.109 mL

Molarity calculator

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Spectrum


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Spectral properties

Excitation (nm)489
Emission (nm)520