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Helixyte™ 14
Equivalent to SYBR 14; 5 mM
Helixyte™ 14 has the same chemical structure of SYBR 14 (SYBR is the trademark of ThermoFisher). It is commonly used to monitor the viability of sperms. The proportion of living sperm in semen can be assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). Helixyte™ 14 is a nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. It stains the nuclei of living sperm bright green. Conversely, PI stains only nonmotile sperm that had lost their membrane integrity. The proportions of living and dead sperm can be determined by first staining with SYBR-14 and PI and then assessing stain uptake by flow cytometry. The proportions of living and dead sperm in mammalian semen can be readily identified through use of dual staining with SYBR-14 and PI and quantified through use of flow cytometry.
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CatalogSize
Price
Quantity
175630.5 mL
Price
 
Physical properties

Molecular weightN/A
SolventDMSO
Spectral properties

Excitation (nm)489
Emission (nm)520
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025