Name: Helixyte™ 14 [Equivalent to SYBR 14] *5 mM*
Brand: AAT Bioquest
SKU: 17563
Price: 213 USD
Availability: InStock

Helixyte™ 14

Equivalent to SYBR 14; 5 mM

Helixyte™ 14 has the same chemical structure of SYBR 14 (SYBR is the trademark of ThermoFisher). It is commonly used to monitor the viability of sperms. The proportion of living sperm in semen can be assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). Helixyte™ 14 is a nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. It stains the nuclei of living sperm bright green. Conversely, PI stains only nonmotile sperm that had lost their membrane integrity. The proportions of living and dead sperm can be determined by first staining with SYBR-14 and PI and then assessing stain uptake by flow cytometry. The proportions of living and dead sperm in mammalian semen can be readily identified through use of dual staining with SYBR-14 and PI and quantified through use of flow cytometry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17563	0.5 mL	Price

References

View all 50 references: Citation Explorer

Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender.

Authors:

Hermansson, Ulrika and Johannisson, Anders and Axnér, Eva

Journal:

Veterinary medicine and science (2021)

Evaluation of the use of SYBR-14 and propidium iodide stain in a fluorescent computer-assisted spermatozoal quantification method in dogs.

Authors:

Watts, John R

Journal:

Reproduction in domestic animals = Zuchthygiene (2021): 89-102

Measurement of membrane integrity in canine spermatozoa using a fluorescent computer-assisted spermatozoal quantification method after SYBR-14/PI staining compared with manual counting after CFDA/PI staining.

Authors:

Watts, John

Journal:

Reproduction in domestic animals = Zuchthygiene (2021)

The impact of five years storage/biobanking at -80°C on mouse spermatozoa fertility, physiology, and function.

Authors:

Raspa, Marcello and Putti, Sabrina and Paoletti, Renata and Barboni, Barbara and Ramal-Sanchez, Marina and Lanuti, Paola and Marchisio, Marco and D'Atri, Mario and Ortolani, Claudio and Papa, Stefano and Valbonetti, Luca and Bernabo, Nicola and Scavizzi, Ferdinando

Journal:

Andrology (2021)

Butaphosphan and Cyanocobalamin Supplementation in Semen Extender on Chilled Boar Sperm Quality and Life Span.

Authors:

Suwimonteerabutr, J and Chumsri, S and Tummaruk, P and Nuntapaitoon, Morakot

Journal:

Frontiers in veterinary science (2020): 592162

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	489
Emission (nm)	520

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 29, 2025