Helixyte™ Green *20X Aqueous PCR Solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | N/A |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 498 |
| Emission (nm) | 522 |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | T |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
Alternative formats
| Helixyte™ Green *10,000X Aqueous PCR Solution* |
Related products
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus, DNA and RNA Quantitation, Enzymes, Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Serology Assays for Antibody Detection, PCR Detection of Viral DNA/RNA
Molecular weight N/A | Excitation (nm) 498 | Emission (nm) 522 |
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to those of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.
Product Family
| Name | Excitation (nm) | Emission (nm) |
| Helixyte™ 14 [Equivalent to SYBR 14] *5 mM* | 489 | 520 |
| Helixyte™ 14 [Equivalent to SYBR 14] | 489 | 520 |
| Indocyanine Green *CAS 3599-32-4* | 789 | 814 |
| Thiolite™ Green | 510 | 525 |
| CytoTell™ Green | 510 | 525 |
| LysoBrite™ Green | 501 | 510 |
| Q4ever™ Green *1250X DMSO Solution* | 503 | 527 |
Images
Figure 1. Quantitative PCR results targeting GAPDH with an input of 100 ng–0.00001 ng cDNA was performed using Helixyte™ Green *20X Aqueous PCR Solution* (Cat No. 17591) and a Fast Advanced Master Mix on an Applied Biosystems® 7500 FAST Real-Time PCR System.
References
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