Helixyte™ Green *20X Aqueous PCR Solution*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 498 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
Helixyte™ Green *10,000X Aqueous PCR Solution* |
Related products
Overview | ![]() ![]() |
See also: Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), PCR Detection of Viral DNA/RNA
Molecular weight N/A | Excitation (nm) 498 | Emission (nm) 522 |
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to those of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.
Product Family
Name | Excitation (nm) | Emission (nm) |
Helixyte™ 14 [Equivalent to SYBR 14] *5 mM* | 489 | 520 |
Helixyte™ 14 [Equivalent to SYBR 14] | 489 | 520 |
Indocyanine Green *CAS 3599-32-4* | 789 | 814 |
Thiolite™ Green | 505 | 524 |
CytoTell™ Green | 510 | 525 |
LysoBrite™ Green | 501 | 510 |
Q4ever™ Green *1250X DMSO Solution* | 503 | 527 |
Droplite™ Green | 421 | 521 |
Images
References
View all 22 references: Citation Explorer
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Journal: Stroke (2012): 524
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Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
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Journal: Lasers Surg Med (2010): 630
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Journal: Lasers Surg Med (2010): 630
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Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
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Journal: J Acupunct Meridian Stud (2008): 29
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Journal: Anal Biochem (2007): 87
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Journal: Anal Biochem (2007): 87
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Journal: Biomacromolecules (2005): 2703
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Journal: Biomacromolecules (2005): 2703
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Journal: Histochem Cell Biol (2001): 293
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Journal: Biochim Biophys Acta (2001): 61
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Journal: Photochem Photobiol (2001): 585
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Journal: Photochem Photobiol (2001): 585
Application notes
PCR Annealing Temperature Optimization
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
What are the generally used detection methods in real time PCR (qPCR)?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?