Helixyte Green (SYBR Green I)

Helixyte™ Green

20X Aqueous PCR Solution

Helixyte™ Green is a nucleic acid binding dye (equivalent to SYBR Green I) that exhibits dramatic fluorescence enhancement upon binding dsDNA, widely used for real-time PCR, gel staining, and DNA quantification.

SYBR® Green I Equivalent: Chemically identical to Thermo Fisher's SYBR® Green I with matching spectral properties (Ex/Em: 498/522 nm) for seamless protocol substitution
Versatile DNA Detection: Fluorogenic intercalating dye that becomes highly fluorescent upon binding to dsDNA, suitable for both gel electrophoresis and real-time qPCR applications
Standard Instrument Compatibility: Optimally excited by 488 nm laser lines and detected in FITC channels, compatible with common fluorescence microscopes and flow cytometers
Enhanced Safety Profile: Lower cell permeability compared to ethidium bromide reduces mutagenic risk, while green excitation eliminates the need for hazardous UV exposure

SYBR Green spectrum. SYBR Green is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 522 nm. Other spectra of interest include: Helixyte™ Green Nucleic Acid Gel Stain, Helixyte™ Green dsDNA, and Q4ever™ Green. Helixyte™ Green belongs to the following categories: Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Nucleic Acid Building Blocks, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), and Single Cell RNA Sequencing (scRNA-seq).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17591	5x1 ml	Price

Overview

SYBR® Green I (Thermo Fisher Scientific) and Helixyte™ Green are chemically equivalent, nucleic acid stains, with an excitation peak at 498 nm and an emission peak at 522 nm. The dye is useful for the detection of double-stranded DNA (dsDNA) in gel electrophoresis and quantitative PCR (qPCR) applications.

Staining mechanism and principle

Helixyte™ Green is an intercalating nucleic acid dye belonging to the cyanine dye family. It is fluorogenic, becoming highly fluorescent upon binding to the minor groove of dsDNA and, to a lesser extent, when bound to single stranded DNA (ssDNA) and RNA. It can be used to stain dsDNA in polyacrylamide and agarose gels in electrophoresis applications. It is also useful for monitoring qPCR, wherein fluorescence measured during the annealing or extension stage is proportional to the amount of PCR product. In these applications, often the dye is provided alongside the master mix.

Excitation / Emission

Helixyte Green and SYBR Green I have the same fluorescence spectrum, with an excitation peak at 498 nm and an emission peak at 522 nm. They are well excited by the 488 nm laser line and can be detected in the FITC channel.

Helixyte/SYBR Green versus Ethidium Bromide (EtBr)

Helixyte™ Green and SYBR Green stains are in principle safer and less mutagenic than EtBr because they are less cell permeable. Additionally, these dyes are experimentally more convenient due to having a green excitation wavelength, which is compatible with common lab instruments, such as the FITC channel on a fluorescence microscope or the 488 nm laser on a flow cytometer. This is in contrast to EtBr, which requires the more dangerous UV excitation wavelength.

SYBR Green I versus SYBR Green II

SYBR Green I has a strong affinity for dsDNA, whereas SYBR Green II has a strong affinity for RNA.

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice

Authors:

Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.

Journal:

Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence

Authors:

Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.

Journal:

Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques

Authors:

van 't Hoff M, Reuter M, Dryden DT, Oheim M.

Journal:

Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians

Authors:

Lee BC, Kim S, Soh KS.

Journal:

J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy

Authors:

Shimizu M, Sasaki S, Kinjo M.

Journal:

Anal Biochem (2007): 87

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

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Page updated on February 12, 2025