logo
AAT Bioquest

Helixyte™ Green Maleimide

Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral and DNA-binding properties as SYBR Green® (SYBR Green® is the trademark of ThermoFisher). AAT Bioquest’s scientists developed Helixyte™ Green maleimide to enable the development of site-specific DNA probes. Helixyte™ Green maleimide is an excellent building block that readily reacts with thiol-containing biological molecules such as peptides, antibodies and thiol-modified oligos. Thus, it can be used to develop a variety of site-directed DNA probes. Helixyte™ Green is an excellent fluorogenic probe for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte™ Green is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays.

Spectrum

References

View all 50 references: Citation Explorer
Fluorescent Techniques for RNA Detection in Nanoparticles.
Authors: Carneiro, Simone P and Müller, Joschka T and Merkel, Olivia M
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 187-203
Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in Escherichia coli.
Authors: López, Isidoro and Otero, Fátima and Fernández, María Del Carmen and Bou, Germán and Gosálvez, Jaime and Fernández, José Luis
Journal: Antibiotics (Basel, Switzerland) (2024)
Construction of a label-free fluorescent biosensor for homogeneous detection of m6A eraser FTO in breast cancer tissues.
Authors: Liu, Ming-Hao and Zhao, Ning-Ning and Yu, Wan-Tong and Qiu, Jian-Ge and Jiang, Bing-Hua and Zhang, Yan and Zhang, Chun-Yang
Journal: Talanta (2024): 125784
Sensitive and rapid analysis of plasmid DNA topology isoforms by capillary gel electrophoresis with laser induced fluorescence in uncoated capillary.
Authors: He, Yan and Orlet, John and Cunio, Olivia and Thompson, Lawrence and Jones, Michael T
Journal: Electrophoresis (2024): 1389-1397
Residual OXPHOS is required to drive primary and metastatic lung tumours in an orthotopic breast cancer model.
Authors: Herst, Patries and Carson, Georgia and Lewthwaite, Danielle and Eccles, David and Schmidt, Alfonso and Wilson, Andrew and Grasso, Carole and O'Sullivan, David and Neuzil, Jiri and McConnell, Melanie and Berridge, Michael
Journal: Frontiers in oncology (2024): 1362786
Page updated on May 19, 2025

Ordering information

Price
Unit size
Catalog Number17614
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1086.06

Solvent

Water

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure