Name: Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*
Brand: AAT Bioquest
SKU: 17950
Price: 516 USD
Availability: InStock

Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright emission detectable with AMCA/Alexa Fluor® 350 filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

Direct labeling of nucleic acid using Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye. DNA ladder was labeled with 50 µM of Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye (Lane 4) and analyzed alongside unlabeled DNA (Lane 1) on 1% agarose DNA gel using gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17950	1 mg	Price

Overview

Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugations are either tedious or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 350 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 350 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright blue fluorescence that can be easily detected with AMCA/Alexa Fluor 350 filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 50 references: Citation Explorer

Visualizing DNA replication by single-molecule analysis of replicated DNA.

Authors:

Madireddy, Advaitha and Gerhardt, Jeannine

Journal:

STAR protocols (2023): 102721

Glass Nanopipette-Based Plasmonic SERS Platform for Single-Cell MicroRNA-21 Sensing during Apoptosis.

Authors:

Wang, Yong and Wang, Dandan and Qi, Guohua and Hu, Ping and Wang, Erkang and Jin, Yongdong

Journal:

Analytical chemistry (2023): 16234-16242

Rapid, inexpensive, sequence-independent fluorescent labeling of phosphorothioate DNA.

Authors:

Satusky, Matthew J and Johnson, Caitlin V and Erie, Dorothy A

Journal:

Biophysical journal (2023): 1211-1218

A Step toward Amino Acid-Labeled DNA Sequencing: Boosting Transmission Sensitivity of Graphene Nanogap.

Authors:

Mittal, Sneha and Pathak, Biswarup

Journal:

ACS applied bio materials (2023): 218-227

Inhibition of N-Acetyltransferase 10 Suppresses the Progression of Prostate Cancer through Regulation of DNA Replication.

Authors:

Ma, Ningning and Liu, Haijing and Wu, Yaqian and Yao, Mengfei and Zhang, Bo

Journal:

International journal of molecular sciences (2022)

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 24, 2026