Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*

Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 757 nm and emission at 779 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright, stable fluorescence detectable with Cy7 filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

Direct labeling of nucleic acid using Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye. DNA ladder was labeled with 100 µM of Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye (Lane 3) and analyzed alongside unlabeled DNA (Lane 1) on 1% agarose DNA gel using gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17970	1 mg	Price

Overview

Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugation techniques are either tedious, ineffective or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 750 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 750 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright NIR and stable fluorescence that can be easily detected with Cy7 filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 50 references: Citation Explorer

Nile blue as reporter dye in salt aggregation based-colorimetric aptasensors for peptide, small molecule and metal ion detection.

Authors:

Chovelon, Benoît and Peyrin, Eric and Ragot, Mailys and Salem, Nassim and Nguyen, Truong Giang and Auvray, Benjamin and Henry, Mickael and Petrillo, Mel-Alexandre and Fiore, Emmanuelle and Bessy, Quentin and Faure, Patrice and Ravelet, Corinne

Journal:

Analytica chimica acta (2023): 340840

Near-Infrared Dye-Aptamer Assay for Small Molecule Detection in Complex Specimens.

Authors:

Jin, Xin and Liu, Yingzhu and Alkhamis, Obtin and Canoura, Juan and Bacon, Adara and Xu, Ruyi and Fu, Fengfu and Xiao, Yi

Journal:

Analytical chemistry (2022): 10082-10090

Detection of small molecules by fluorescence intensity using single dye labeled aptamers and quencher transition metal ions.

Authors:

Billet, Blandine and Chovelon, Benoit and Fiore, Emmanuelle and Faure, Patrice and Ravelet, Corinne and Peyrin, Eric

Journal:

Biosensors & bioelectronics (2022): 114091

Two-dimensional liquid chromatography coupled to mass spectrometry for impurity analysis of dye-conjugated oligonucleotides.

Authors:

Koshel, Brooke and Birdsall, Robert and Chen, Weibin

Journal:

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2020): 121906

Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement.

Authors:

Botequim, David and Silva, Inês I R and Serra, Sofia G and Melo, Eduardo P and Prazeres, Duarte M F and Costa, Sílvia M B and Paulo, Pedro M R

Journal:

Nanoscale (2020): 6334-6345

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (260 nm)	0.044
Correction factor (280 nm)	0.039
Correction factor (565 nm)	0.0250
Correction factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000 ¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026