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Hoechst 33258 *20 mM solution in water*

Chemical structure for Hoechst 33258 *20 mM solution in water*
Chemical structure for Hoechst 33258 *20 mM solution in water*
Ordering information
Price ()
Catalog Number17525
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight533.88
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)460001
Excitation (nm)352
Emission (nm)454
Quantum yield0.03401
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
23491-45-4
Molecular weight
533.88
Extinction coefficient (cm -1 M -1)
460001
Excitation (nm)
352
Emission (nm)
454
Quantum yield
0.03401
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. These dyes can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 33258 *20 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM187.308 µL936.54 µL1.873 mL9.365 mL18.731 mL
5 mM37.462 µL187.308 µL374.616 µL1.873 mL3.746 mL
10 mM18.731 µL93.654 µL187.308 µL936.54 µL1.873 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)460001
Excitation (nm)352
Emission (nm)454
Quantum yield0.03401

Citations


View all 2 citations: Citation Explorer
Protective Effects of Crataegus pinnatifida Extracts and Crataegolic Acid Against Neurotoxicity of Paraquat in PC12 Cells.
Authors: Chang, Chi-Sen and Shen, Yuh-Chiang and Juan, Chi-Wen and Chang, Chia-Lin and Lin, Po-Kai
Journal: Current Topics in Nutraceutical Research (2022)
Effects of cyclosporin A pre-treatment combined with etomidate post-treatment on lung injury induced by limb ischemia-reperfusion in rats
Authors: Zou, Haibo and Sun, Xiaofeng
Journal: Journal of International Medical Research (2020): 0300060520934627

References


View all 42 references: Citation Explorer
DNA sequence recognition by Hoechst 33258 conjugates of hairpin pyrrole/imidazole polyamides
Authors: Correa BJ, Canzio D, Kahane AL, Reddy PM, Bruice TC.
Journal: Bioorg Med Chem Lett (2006): 3745
Recognition of B-DNA by neomycin--Hoechst 33258 conjugates
Authors: Willis B, Arya DP.
Journal: Biochemistry (2006): 10217
Design of new bidentate ligands constructed of two Hoechst 33258 units for discrimination of the length of two A3T3 binding motifs
Authors: Tanada M, Tsujita S, Sasaki S.
Journal: J Org Chem (2006): 125
The Hoechst 33258 Covalent Dimer Covers a Total Turn of the Double-stranded DNA
Authors: Streltsov SA, Gromyko AV, Oleinikov VA, Zhuze AL.
Journal: J Biomol Struct Dyn (2006): 285
Amine terminated G-6 PAMAM dendrimer and its interaction with DNA probed by Hoechst 33258
Authors: Choi YS, Cho TS, Kim JM, Han SW, Kim SK.
Journal: Biophys Chem (2006): 142
Ligands with affinity to specific sequence of DNA base pairs. XII. The synthesis and cytological studies of dimeric Hoechst 33258 molecules
Authors: Gromyko AV, Popov KV, Mozoleva AP, Strel'tsov SA, Grokhovskii SL, Oleinikov VA, Zhuze AL.
Journal: Bioorg Khim (2005): 385
The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression
Authors: Li L, Yang L, Kotin RM.
Journal: J Gene Med (2005): 420
Unique cooperative binding interaction observed between a minor groove binding Pt antitumor agent and Hoechst dye 33258
Authors: Harris A, Qu Y, Farrell N.
Journal: Inorg Chem (2005): 1196
Synthesis, DNA binding, topoisomerase inhibition and cytotoxic properties of 2-chloroethylnitrosourea derivatives of hoechst 33258
Authors: Bielawski K, Bielawska A, Anchim T, Wolczynski S.
Journal: Biol Pharm Bull (2005): 1004
Association of the minor groove binding drug Hoechst 33258 with d(CGCGAATTCGCG)2: volumetric, calorimetric, and spectroscopic characterizations
Authors: Han F, Taulier N, Chalikian TV.
Journal: Biochemistry (2005): 9785