Name: iFluor® 440 Styramide
Brand: AAT Bioquest
SKU: 44900
Price: 322 USD
Availability: InStock

iFluor® 440 Styramide

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 440 Styramide is a new unique PSA™ reagent for multicolor application with our existing PSA™ and TSA reagents. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA™ reagents for multicolor applications.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong> ™</strong> amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 440 Styramide™ (Cat#44900).

Protocol

SDS

Catalog	Size	Price	Quantity
44900	100 Slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 440 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	131.415 µL	657.073 µL	1.314 mL	6.571 mL	13.141 mL
5 mM	26.283 µL	131.415 µL	262.829 µL	1.314 mL	2.628 mL
10 mM	13.141 µL	65.707 µL	131.415 µL	657.073 µL	1.314 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	760.95 g/mol	=	mL	x	mM	=	mmol

References

View all 5 references: Citation Explorer

Using participatory action research to improve immunization utilization in areas with pockets of unimmunized children in Nigeria.

Authors:

Akwataghibe, Ngozi N and Ogunsola, Elijah A and Popoola, Oluwafemi A and Agbo, Adanna I and Dieleman, Marjolein A

Journal:

Health research policy and systems (2021): 88

A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.

Authors:

Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A

Journal:

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782

Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification-Fluorescent In Situ Hybridization.

Authors:

Riou, Virginie and Périot, Marine and Biegala, Isabelle C

Journal:

Frontiers in microbiology (2017): 854

CARD-FISH for environmental microorganisms: technical advancement and future applications.

Authors:

Kubota, Kengo

Journal:

Microbes and environments (2013): 3-12

Detection of prokaryotic cells with fluorescence in situ hybridization.

Authors:

Zwirglmaier, Katrin

Journal:

Methods in molecular biology (Clifton, N.J.) (2010): 349-62

Physical properties

Molecular weight	760.95
Solvent	DMSO

Spectral properties

Absorbance (nm)	430
Correction factor (260 nm)	0.352
Correction factor (280 nm)	0.229
Extinction coefficient (cm ^-1 M ^-1)	40000 ¹
Excitation (nm)	434
Emission (nm)	480
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	DAPI/FITC filter set
Emission	DAPI/FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)

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Page updated on December 26, 2025