iFluor® 440 Styramide
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 760.95 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 430 |
Correction Factor (260 nm) | 0.352 |
Correction Factor (280 nm) | 0.229 |
Extinction coefficient (cm -1 M -1) | 400001 |
Excitation (nm) | 434 |
Emission (nm) | 480 |
Quantum yield | 0.671 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 760.95 | Absorbance (nm) 430 | Correction Factor (260 nm) 0.352 | Correction Factor (280 nm) 0.229 | Extinction coefficient (cm -1 M -1) 400001 | Excitation (nm) 434 | Emission (nm) 480 | Quantum yield 0.671 |
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 440 Styramide is a new unique PSA reagent for multicolor application with our existing PSA and TSA reagents. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA reagents for multicolor applications.
Platform
Fluorescence microscope
Excitation | DAPI/FITC filter set |
Emission | DAPI/FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Protocol Summary
- Fix/permeabilize/block cells or tissue
- Add primary antibody in blocking buffer
- Add HRP-conjugated secondary antibody
- Prepare Styramide™ working solution and apply in cells or tissue for 5-10 minutes at room temperature
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note Make single use aliquots, and store unused 100X stock solution at 2-8 oC in dark place.
Note Prepare the 100X H2O2 solution fresh on the day of use.
1. iFluor™ 440 Styramide stock solution (100X)
Add 100 µL of DMSO into the vial of iFluor™ 440 Styramide conjugate to make 100X Styramide stock solution.Note Make single use aliquots, and store unused 100X stock solution at 2-8 oC in dark place.
2. H2O2 stock solution
Add 10 µL of 3% hydrogen peroxide (Not provided) to 90 µL of ddH2O.Note Prepare the 100X H2O2 solution fresh on the day of use.
PREPARATION OF WORKING SOLUTION
1. iFluor™ 440 Styramide working solution (1X)
Every 1 mL of Reaction Buffer requires 10 µL of Styramide stock solution and 10 µL of H2O2 stock solution.Note The Styramide provided is enough for 100 tests based on 100 µL of Styramide working solution needed per coverslip or per well in a 96-well microplate.
Note The Styramide working solution must be used within 2 hours after preparation and avoid direct exposure to light.
2. Secondary antibody-HRP working solution
Make appropriate concentration of secondary antibody-HRP working solution as per the manufacturer's recommendations.SAMPLE EXPERIMENTAL PROTOCOL
This protocol is applicable for both cells and tissues staining.
Protocol can be found at
https://www.aatbio.com/resources/guides/paraffin-embedded-tissue-immunohistochemistry-protocol.html
Cell fixation and permeabilization
- Fix the cells or tissue with 3.7% formaldehyde or paraformaldehyde, in PBS at room temperature for 20 minutes.
- Rinse the cells or tissue with PBS twice.
- Permeabilize the cells with 0.1% Triton X-100 solution for 1-5 minutes at room temperature.
- Rinse the cells or tissue with PBS twice.
Tissue fixation, deparaffinization and rehydration
Deparaffinize and dehydrate the tissue according to the standard IHC protocols. Perform antigen retrieval with preferred specific solution/protocol as needed.Protocol can be found at
https://www.aatbio.com/resources/guides/paraffin-embedded-tissue-immunohistochemistry-protocol.html
Peroxidase labeling
- Optional: Quench endogenous peroxidase activity by incubating cell or tissue sample in peroxidase quenching solution (such as 3% hydrogen peroxide) for 10 minutes. Rinse with PBS twice at room temperature.
- Optional: If using HRP-conjugated streptavidin, it is advisable to block endogenous biotins by biotin blocking buffer.
- Block with preferred blocking solution (such as PBS with 1% BSA) for 30 minutes at 4 °C.
- Remove blocking solution and add primary antibody diluted in recommended antibody diluent for 60 minutes at room temperature or overnight at 4 °C.
- Wash with PBS three times for 5 minutes each.
- Apply 100 µL of secondary antibody-HRP working solution to each sample and incubate for 60 minutes at room temperature.
Note Incubation time and concentration can be varied depending on the signal intensity. - Wash with PBS three times for 5 minutes each.
Styramide labeling
- Prepare and apply 100 µL of Styramide working solution to each sample and incubate for 5-10 minutes at room temperature.
Note If you observe non-specific signal, you can shorten the incubation time with Styramide. You should optimize the incubation period using positive and negative control samples at various incubation time points. Or you can use lower concentration of Styramide in the working solution. - Rinse with PBS three times.
Counterstain and fluorescence imaging
- Counterstain the cell or tissue samples as needed. AAT provides a series of nucleus counterstain reagents as listed in Table 1. Follow the instruction provided with the reagents.
- Mount the coverslip using a mounting medium with anti-fading properties.
- Use the appropriate filter set to visualize the signal from the Styramide labeling.
Cat# | Product Name | Ex/Em (nm) |
17548 | Nuclear Blue™ DCS1 | 350/461 |
17550 | Nuclear Green™ DCS1 | 503/526 |
17551 | Nuclear Orange™ DCS1 | 528/576 |
17552 | Nuclear Red™ DCS1 | 642/660 |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 440 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 131.415 µL | 657.073 µL | 1.314 mL | 6.571 mL | 13.141 mL |
5 mM | 26.283 µL | 131.415 µL | 262.829 µL | 1.314 mL | 2.628 mL |
10 mM | 13.141 µL | 65.707 µL | 131.415 µL | 657.073 µL | 1.314 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 430 |
Correction Factor (260 nm) | 0.352 |
Correction Factor (280 nm) | 0.229 |
Extinction coefficient (cm -1 M -1) | 400001 |
Excitation (nm) | 434 |
Emission (nm) | 480 |
Quantum yield | 0.671 |
Product Family
Images

Figure 1. Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA ™ amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by iFluor® 440 Styramide™ (Cat#44900).

Figure 2. Fixed and permeabilized HeLa cells were incubated with rabbit anti-tubulin antibody, then labeled with HRP-labeled Goat anti-Rabbit IgG (Cat No. 16793), and detected using iFluor® 440 styramide (Cat No. 44900). Images were captured on a fluorescence microscope equipped with a DAPI filter set.
References
View all 5 references: Citation Explorer
Using participatory action research to improve immunization utilization in areas with pockets of unimmunized children in Nigeria.
Authors: Akwataghibe, Ngozi N and Ogunsola, Elijah A and Popoola, Oluwafemi A and Agbo, Adanna I and Dieleman, Marjolein A
Journal: Health research policy and systems (2021): 88
Authors: Akwataghibe, Ngozi N and Ogunsola, Elijah A and Popoola, Oluwafemi A and Agbo, Adanna I and Dieleman, Marjolein A
Journal: Health research policy and systems (2021): 88
A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.
Authors: Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782
Authors: Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782
Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification-Fluorescent In Situ Hybridization.
Authors: Riou, Virginie and Périot, Marine and Biegala, Isabelle C
Journal: Frontiers in microbiology (2017): 854
Authors: Riou, Virginie and Périot, Marine and Biegala, Isabelle C
Journal: Frontiers in microbiology (2017): 854
CARD-FISH for environmental microorganisms: technical advancement and future applications.
Authors: Kubota, Kengo
Journal: Microbes and environments (2013): 3-12
Authors: Kubota, Kengo
Journal: Microbes and environments (2013): 3-12
Detection of prokaryotic cells with fluorescence in situ hybridization.
Authors: Zwirglmaier, Katrin
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 349-62
Authors: Zwirglmaier, Katrin
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 349-62