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iFluor® 488 Anti-human CD4 Antibody *RPA-T4*

RPA-T4 is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (sometimes called T4 or Leu3a) is a 55 kD transmembrane glycoprotein that is expressed on the surface of cells such as granulocytes, T cells and macrophages. CD4 acts in vital cellular pathways, for instance, the T cell receptor signaling pathway, interleukin-15-mediated signaling pathway and enzyme linked receptor protein signaling pathway. Furthermore, in many organisms, it promotes interleukin-2 biosynthetic process, upregulates peptidyl-tyrosine phosphorylation and acts to positively regulate I-kappaB kinase/NF-kappaB signaling. CD4 has been associated with key biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane, and is associated with a variety of biologically interesting macromolecules/ligands, namely, gp120 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd4 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on November 12, 2024

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1004105010041051
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesLeu-3; T4
CloneRPA-T4
Hostmouse
Isotypemouse IgG1
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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