iFluor® 488 Anti-human CD4 Antibody *RPA-T4*

RPA-T4 is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (sometimes called T4 or Leu3a) is a 55 kD transmembrane glycoprotein that is expressed on the surface of cells such as granulocytes, T cells and macrophages. CD4 acts in vital cellular pathways, for instance, the T cell receptor signaling pathway, interleukin-15-mediated signaling pathway and enzyme linked receptor protein signaling pathway. Furthermore, in many organisms, it promotes interleukin-2 biosynthetic process, upregulates peptidyl-tyrosine phosphorylation and acts to positively regulate I-kappaB kinase/NF-kappaB signaling. CD4 has been associated with key biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane, and is associated with a variety of biologically interesting macromolecules/ligands, namely, gp120 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).