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iFluor® 488 Anti-human CD4 Antibody *SK3*

SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen. CD4 (alternatively called T4 or Leu3a) is a 55 kD member of the Ig superfamily that is located on the surface of cells such as macrophages, T cells and granulocytes. CD4 has been associated with important biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane. Furthermore, it is involved with vital cellular pathways, for example, the T cell receptor signaling pathway, enzyme linked receptor protein signaling pathway and cytokine-mediated signaling pathway. In some organisms, CD4 acts to positively regulate peptidyl-tyrosine phosphorylation, is an enhancer of transcription, DNA-templated and is a positive regulator of ERK1 and ERK2 cascade. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as MHC Class II, gp120, IL-16 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 509/24 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd4 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on December 1, 2024

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1004205010042051
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesLeu-3; T4
CloneSK3
Hostmouse
IsotypeMouse IgG1, κ
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Detection of CD4 in human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with mouse anti-human CD4 iFluor®488 conjugated monoclonal antibody. The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the FITC channel.
Detection of CD4 in human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with mouse anti-human CD4 iFluor®488 conjugated monoclonal antibody. The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the FITC channel.
Detection of CD4 in human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with mouse anti-human CD4 iFluor®488 conjugated monoclonal antibody. The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the FITC channel.
Flow cytometric analysis of human peripheral blood lymphocytes stained with CD4 (clone SK3) iFluor® 488 (red filled histogram) or CD4 (clone SK3) Alexa Fluor® 488 (green filed histogram). The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the FITC channel.
Top) The Spectral pattern was generated using a 4-laser spectral cytometer. Four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, which, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the iFluor® 488 B2-A channel.
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