Name: iFluor® 488 Anti-human CD8 Antibody *OKT-8*
Brand: AAT Bioquest
SKU: 10082050
Availability: InStock

iFluor® 488 Anti-human CD8 Antibody

OKT-8

OKT-8 is an anti-human monoclonal antibody that forms an immune complex with the CD8a antigen. CD8a (alternatively called T8 or MAL) is a 32 - 34 kD transmembrane glycoprotein that is located on the surface of cells such as T cells. CD8 is a component of essential cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 120000 publications in the last decade. CD8a has a variety of applications in immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 527/32 nm bandpass filter (for example, as in the BD FACSVerse™).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10082050	100 tests	Price
10082051	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd8a antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	T8; Leu2
Clone	OKT-8
Host	mouse
Isotype	mouse igg2a
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 19, 2025