Products
Technologies
Applications
Services
Resources
Selection Guides
About
iFluor® 488 Anti-mouse CD19 Antibody
1D3
1D3 is an anti-mouse monoclonal antibody that is specific for the CD19 antigen. CD19 (sometimes referred to as B4) is a 95 kD transmembrane glycoprotein that is expressed on the surface of cells like B cells and stem cells. CD19 plays a role in essential cellular pathways, namely, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. In addition, in some organisms, it is an enhancer of release of sequestered calcium ion into cytosol, is a promoter of protein kinase B signaling and is a positive regulator of phosphatidylinositol 3-kinase activity. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like CD225, CD81, PI3-kinase and fyn. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 has been widely used in costimulatory molecules and immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant X).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10194050 | 100 tests | Price | |
| 10194051 | 500 tests | Price |
Antibody properties
| Other names | B4 |
| Clone | 1D3 |
| Host | rat |
| Isotype | Rat IgG2a, κ |
| Reactivity | mouse |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.21 |
| Correction factor (280 nm) | 0.11 |
| Extinction coefficient (cm -1 M -1) | 75000 1 |
| Excitation (nm) | 491 |
| Emission (nm) | 516 |
| Quantum yield | 0.9 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 19, 2025