Name: iFluor® 532 Anti-human CD73 Antibody *AD2*
Brand: AAT Bioquest
SKU: 10730070
Availability: InStock

iFluor® 532 Anti-human CD73 Antibody

AD2

AD2 is an anti-human monoclonal antibody that is specific for the CD73 antigen. CD73 (sometimes called NT5E or Ecto-5'-nucleotidase) is a 70 kD transmembrane protein that is found on the surface of cells such as epithelial cells, endothelial cells and stem cells. CD73 is associated with a variety of biologically interesting macromolecules/ligands, for instance, AMP. CD73 is a moderately popular antibody target, with over 11000 publications in the last decade. CD73 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells. This antibody was purified through affinity chromatography and conjugated to iFluor® 532 (ex/em = 537/560 nm). It is compatible with the 532 nm laser and 575/36 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Protocol

COA

Catalog	Size	Price	Quantity
10730070	100 tests	Price
10730071	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd73 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	NT5E; Ecto-5'-nucleotidase
Clone	AD2
Host	mouse
Isotype	Mouse IgG1 kappa
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 29, 2025