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iFluor® 568 Anti-human CD73 Antibody *AD2*

AD2 is an anti-human monoclonal antibody that is specific for the CD73 antigen. CD73 (sometimes called NT5E or Ecto-5'-nucleotidase) is a 70 kD transmembrane protein that is found on the surface of cells such as epithelial cells, endothelial cells and stem cells. CD73 is associated with a variety of biologically interesting macromolecules/ligands, for instance, AMP. CD73 is a moderately popular antibody target, with over 11000 publications in the last decade. CD73 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells. This antibody was purified through affinity chromatography and conjugated to iFluor® 568 (ex/em = 568/587 nm). It is compatible with the 561 nm laser and 577/35 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd73 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on September 16, 2024

Ordering information

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Additional ordering information

Telephone1-800-990-8053
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Antibody properties

Other namesNT5E; Ecto-5'-nucleotidase
CloneAD2
Hostmouse
IsotypeMouse IgG1 kappa
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.34

Correction Factor (280 nm)

0.15

Extinction coefficient (cm -1 M -1)

1000001

Excitation (nm)

568

Emission (nm)

587

Quantum yield

0.571

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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