iFluor® 820 Anti-human CD73 Antibody *AD2*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Spectral properties
| Correction Factor (260 nm) | 0.11 |
| Correction Factor (280 nm) | 0.16 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 822 |
| Emission (nm) | 850 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, Cluster of Differentiation (CD Antibodies), Immunohistochemistry (IHC)
Other names NT5E; Ecto-5'-nucleotidase | Clone AD2 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.11 | Correction Factor (280 nm) 0.16 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 822 | Emission (nm) 850 |
AD2 is an anti-human monoclonal antibody that is specific for the CD73 antigen. CD73 (sometimes called NT5E or Ecto-5'-nucleotidase) is a 70 kD transmembrane protein that is found on the surface of cells such as epithelial cells, endothelial cells and stem cells. CD73 is associated with a variety of biologically interesting macromolecules/ligands, for instance, AMP. CD73 is a moderately popular antibody target, with over 11000 publications in the last decade. CD73 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells. This antibody was purified through affinity chromatography and conjugated to iFluor® 820 (ex/em = 822/850 nm).
Spectrum
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Spectral properties
| Correction Factor (260 nm) | 0.11 |
| Correction Factor (280 nm) | 0.16 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 822 |
| Emission (nm) | 850 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd73 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.