iFluor® A7 Anti-human CD127 Antibody *A019D5*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 759 |
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 275000 |
Excitation (nm) | 764 |
Emission (nm) | 782 |
Quantum yield | 0.1 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names IL-7Rα; IL-7R | Clone A019D5 | Host mouse | Isotype mouse IgG1 | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 759 | Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.03 | Extinction coefficient (cm -1 M -1) 275000 | Excitation (nm) 764 | Emission (nm) 782 | Quantum yield 0.1 |
A019D5 is an anti-human monoclonal antibody that targets the CD127 antigen. CD127 (sometimes called IL7R or IL-7Rα) is a 60 - 90 kD single-pass type i membrane protein that is located on the surface of cells such as stem cells and T cells. In some organisms, CD127 is an inhibitor of T cell mediated cytotoxicity, acts to positively regulate cell population proliferation and is an enhancer of gene expression. Also, it is involved with important cellular pathways, for instance, the cell surface receptor signaling pathway, interleukin-7-mediated signaling pathway and positive regulation of receptor signaling pathway via STAT. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as IL-7, PI3-kinase and CD132. CD127 is a fairly uncommon antibody target, with a little more than 7000 publications in the last decade. Even still, CD127 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® A7.
Spectrum
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Spectral properties
Absorbance (nm) | 759 |
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 275000 |
Excitation (nm) | 764 |
Emission (nm) | 782 |
Quantum yield | 0.1 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd127 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
What are the commonly used CD makers for the analysis of Tregs by flow cytometry?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?