iFluor® A7 Anti-human CD127 Antibody *A019D5*

iFluor® A7 Anti-human CD127 Antibody

A019D5

A019D5 is an anti-human monoclonal antibody that targets the CD127 antigen. CD127 (sometimes called IL7R or IL-7Rα) is a 60 - 90 kD single-pass type i membrane protein that is located on the surface of cells such as stem cells and T cells. In some organisms, CD127 is an inhibitor of T cell mediated cytotoxicity, acts to positively regulate cell population proliferation and is an enhancer of gene expression. Also, it is involved with important cellular pathways, for instance, the cell surface receptor signaling pathway, interleukin-7-mediated signaling pathway and positive regulation of receptor signaling pathway via STAT. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as IL-7, PI3-kinase and CD132. CD127 is a fairly uncommon antibody target, with a little more than 7000 publications in the last decade. Even still, CD127 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® A7.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
112700S0	100 tests	Price
112700S1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd127 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	IL-7Rα, IL-7R
Clone	A019D5
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	759
Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	275000
Excitation (nm)	764
Emission (nm)	782
Quantum yield	0.1

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 5, 2024