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JC-10 *Superior alternative to JC-1*

Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be an alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1. Interestingly the performance of JC-10 is quite cell line-dependent. Our JC-10 is conveniently provided in DMSO solution at ~3 mM concentration (2 mg/mL).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2*5155301950001

Citations

View all 96 citations: Citation Explorer
MT2A Promotes Angiogenesis in Chronically Ischemic Brains through a Copper--Mitochondria Regulatory Mechanism
Authors: Mo, Ni and Tai, Chuyang and Yang, Yang and Ling, Cong and Zhang, Baoyu and Wei, Lei and Wang, Hui and Chen, Chuan and others,
Journal: (2024)
Lithocholic Acid’s Ionic Compounds as Promising Antitumor Agents: Synthesis and Evaluation of the Production of Reactive Oxygen Species (ROS) in Mitochondria
Authors: Chobanov, Nuri M and Dzhemileva, Lilya U and Dzhemilev, Usein M and D’yakonov, Vladimir A
Journal: Antioxidants (2024): 1448
Mechanisms of H2pmen-Induced Cell Death: Necroptosis and Apoptosis in MDA Cells, Necrosis in MCF7 Cells.
Authors: Ntanzi, Nosipho and Khan, Rene B and Nxumalo, Mthokozisi B and Kumalo, Hezekiel M
Journal: Heliyon (2024)
Anticancer activity of salinomycin quaternary phosphonium salts
Authors: J{\k{e}}drzejczyk, Marta and Sulik, Micha{\l} and Mielczarek-Puta, Magdalena and Lim, Gwan Yong and Podsiad, Ma{\l}gorzata and Hoser, Jakub and Bednarczyk, Piotr and Struga, Marta and Huczy{\'n}ski, Adam
Journal: European Journal of Medicinal Chemistry (2024): 117055
Self-Assembled Nanocarriers of Synthetic and Natural Plasmalogens for Potential Nanomedicine Development
Authors: Wu, Yu and Angelov, Borislav and Deng, Yuru and Fujino, Takehiko and Hossain, Md Shamim and Bizien, Thomas and Angelova, Angelina
Journal: Advanced Therapeutics (2024): 2400093

References

View all 6 references: Citation Explorer
Tissue plasminogen activator regulates Purkinje neuron development and survival
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
5,6-Dimethylxanthenone-4-acetic Acid (DMXAA) Activates Stimulator of Interferon Gene (STING)-dependent Innate Immune Pathways and Is Regulated by Mitochondrial Membrane Potential
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Application of a homogenous membrane potential assay to assess mitochondrial function
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Human iPS-derived Cardiomyocytes for Cardiotoxicity Screening
Authors: , undefined
Journal: SBS Molecular Devices (2011)
Analyzing Cellular Apoptosis Through Monitoring Mitochondrial Membrane Potential Changes with JC-10
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
Page updated on December 6, 2024

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Catalog Number22204
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Physical properties

Molecular weight

583.34

Solvent

DMSO

Spectral properties

Excitation (nm)

508

Emission (nm)

524

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10&trade; and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 &micro;M) for 4 hours, JC-1 and JC-10&trade; dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10&trade; were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10&trade; and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 &micro;M) for 4 hours, JC-1 and JC-10&trade; dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10&trade; were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10&trade; and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 &micro;M) for 4 hours, JC-1 and JC-10&trade; dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10&trade; were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).