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JC-10 *Superior alternative to JC-1*

Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight583.34
SolventDMSO
Spectral properties
Excitation (nm)508
Emission (nm)524
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
583.34
Excitation (nm)
508
Emission (nm)
524
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be an alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1. Interestingly the performance of JC-10 is quite cell line-dependent. Our JC-10 is conveniently provided in DMSO solution at ~3 mM concentration (2 mg/mL).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of JC-10 *Superior alternative to JC-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.427 µL857.133 µL1.714 mL8.571 mL17.143 mL
5 mM34.285 µL171.427 µL342.853 µL1.714 mL3.429 mL
10 mM17.143 µL85.713 µL171.427 µL857.133 µL1.714 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)508
Emission (nm)524

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2*5155301950001

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Citations


View all 52 citations: Citation Explorer
Sediment pollutant exposures caused hepatotoxicity and disturbed glycogenesis
Authors: Lin, Meng-Wei and Yu, Xin-Ru and Chen, Jai-Yu and Wei, Yu-Shan and Chen, Hsin-Yi and Tsai, Yi-Ting and Lin, Li-Hsun and Liao, En-Chi and Kung, Hsiang-Yu and Young, Shuh-Sen and others,
Journal: Ecotoxicology and Environmental Safety (2023): 114559
Impact of Organelle Transport Deficits on Mitophagy and Autophagy in Niemann--Pick Disease Type C
Authors: Liedtke, Maik and V{\"o}lkner, Christin and Hermann, Andreas and Frech, Moritz J
Journal: Cells (2022): 507
REC drives recombination to repair double-strand breaks in animal mtDNA
Authors: Klucnika, Anna and Mu, Peiqiang and Jezek, Jan and McCormack, Matthew and Di, Ying and Bradshaw, Charles R and Ma, Hansong
Journal: Journal of Cell Biology (2022): e202201137
Garlic exosome-like nanoparticles reverse high-fat diet induced obesity via the gut/brain axis
Authors: Sundaram, Kumaran and Mu, Jingyao and Kumar, Anil and Behera, Jyotirmaya and Lei, Chao and Sriwastva, Mukesh K and Xu, Fangyi and Dryden, Gerald W and Zhang, Lifeng and Chen, ShaoYu and others,
Journal: Theranostics (2022): 1220
Kinase inhibitor-induced cardiotoxicity assessed in vitro with human pluripotent stem cell derived cardiomyocytes
Authors: Xian, Hai-Qing and Blanco, Carmina and Bonham, Kristina and Snodgrass, H Ralph
Journal: Toxicology and applied pharmacology (2022): 115886
DNA repair proteins as the targets for paroxetine to induce cytotoxicity in gastric cancer cell AGS
Authors: Liu, Bang-Hung and Yuan, Tein-Ming and Huang, Chih-Jou and Hsu, Duan-Ting and Chen, Shi-Wen and Hsiao, Nai-Wan and Lin, Sheng-Chih and Wu, Shu-Wan and Lin, Yi-Mei J and Chuang, Show-Mei
Journal: American journal of cancer research (2022): 1465
Tuning the Hydrophobicity of Pyridinium-Based Probes to Realize the Mitochondria-Targeted Photodynamic Therapy and Mitophagy Tracking
Authors: Zhang, Yuyang and Wang, Lianke and Rao, Qingpeng and Bu, Yingcui and Xu, Tianren and Zhu, Xiaojiao and Zhang, Jie and Tian, Yupeng and Zhou, Hongping
Journal: Sensors and Actuators B: Chemical (2020): 128460
Cellular $\alpha$-synuclein pathology is associated with bioenergetic dysfunction in Parkinson’s iPSC-derived dopamine neurons
Authors: Zambon, Federico and Cherubini, Marta and Fernandes, Hugo JR and Lang, Charmaine and Ryan, Brent J and Volpato, Viola and Bengoa-Vergniory, Nora and Vingill, Siv and Attar, Moustafa and Booth, Heather DE and others,
Journal: Human molecular genetics (2019): 2001--2013
Quantitative proteomics in Friedreich's ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis
Authors: T{\'e}lot, Lor{\`e}ne and Rousseau, Elodie and Lesuisse, Emmanuel and Garcia, Camille and Morlet, Bastien and L{\'e}ger, Thibaut and Camadro, Jean-Michel and Serre, Val{\'e}rie
Journal: Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2018): 997--1009
Role of Mcl-1 in regulation of cell death in human induced pluripotent stem cell-derived cardiomyocytes in vitro
Authors: Guo, Liang and Eldridge, S and y , undefined and Furniss, Michael and Mussio, Jodie and Davis, Myrtle
Journal: Toxicology and Applied Pharmacology (2018)

References


View all 6 references: Citation Explorer
Tissue plasminogen activator regulates Purkinje neuron development and survival
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
5,6-Dimethylxanthenone-4-acetic Acid (DMXAA) Activates Stimulator of Interferon Gene (STING)-dependent Innate Immune Pathways and Is Regulated by Mitochondrial Membrane Potential
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Application of a homogenous membrane potential assay to assess mitochondrial function
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Human iPS-derived Cardiomyocytes for Cardiotoxicity Screening
Authors: , undefined
Journal: SBS Molecular Devices (2011)
Analyzing Cellular Apoptosis Through Monitoring Mitochondrial Membrane Potential Changes with JC-10
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
iCell&reg; Cardiomyocytes: Assaying Mitochondrial Membrane Potential
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf