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JC-10 *Superior alternative to JC-1*

Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
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Physical properties
Molecular weight583.34
SolventDMSO
Spectral properties
Excitation (nm)508
Emission (nm)524
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
583.34
Excitation (nm)
508
Emission (nm)
524
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be an alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1. Interestingly the performance of JC-10 is quite cell line-dependent. Our JC-10 is conveniently provided in DMSO solution at ~3 mM concentration (2 mg/mL).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of JC-10 *Superior alternative to JC-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.427 µL857.133 µL1.714 mL8.571 mL17.143 mL
5 mM34.285 µL171.427 µL342.853 µL1.714 mL3.429 mL
10 mM17.143 µL85.713 µL171.427 µL857.133 µL1.714 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)508
Emission (nm)524

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2*5155301950001

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Citations


View all 78 citations: Citation Explorer
Synthesis and Biological Activity of 2-Chloro-8-methoxy-5-methyl-5 H-indolo [2, 3-b] Quinoline for the Treatment of Colorectal Cancer by Modulating PI3K/AKT/mTOR Pathways
Authors: Ma, Yunhao and Zhu, Hongmei and Jiang, Xinrong and Zhou, Zhongkun and Zhou, Yong and Tian, Yanan and Tu, Lixue and Lu, Juan and Niu, Yuqing and Du, Liqian and others,
Journal: ACS Omega (2024)
Green synthesis and characterization of AgNPs, liposomal loaded AgNPs and ZnPcS4 photosensitizer for enhanced photodynamic therapy effects in MCF-7 breast cancer cells
Authors: Chota, Alexander and Abrahamse, Heidi and George, Blassan P
Journal: Photodiagnosis and Photodynamic Therapy (2024): 104252
Xiaohuang Lin1, Jian Zhang1, 2, Zekai Wu1, Yuan Shi3, Mengting Chen1, Maodong Li4, Hong Hu2, Kun Tian1, Xiaoqi Lv5, Chutao Li1, Yang Liu1, Xinyue Gao1, Qiaomei Yang6, Kunqi Chen1* and An Zhu1
Authors: Lin, X and Zhang, J and Wu, Z and Shi, Y and Chen, M and Li, M and Hu, H and Tian, K and Lv, X and Li, C and others,
Journal: (2024)
Chlorogenic Acid as a Potential Therapeutic Agent for Cholangiocarcinoma
Authors: Liang, Jiabao and Wen, Tong and Zhang, Xiaojian and Luo, Xiaoling
Journal: Pharmaceuticals (2024): 794
Effect of autologous platelet-rich plasma on the fertility and quality of cryopreserved buffalo bull semen: a comparative study using OptiXcell{\textregistered} and tris egg yolk extenders
Authors: Salama, Maha S and Ashour, Mohey A and Taher, Ehab S and Rashed, Fatema and Ibrahim, Ibrahim M and El-Nablaway, Mohammad and Ibrahim, Ateya Megahed and Mihaela, Ostan and Olga, Rada and Mohammed, Nourelhuda A and others,
Journal: BMC Veterinary Research (2024): 1--10
The activation of AMPK/PGC-1$\alpha$/GLUT4 signaling pathway through early exercise improves mitochondrial function and mitigates ischemic brain damage
Authors: Wu, Xinyue and Li, Chen and Ke, Changkai and Huang, Chuan and Pan, Bingchen and Wan, Chunxiao
Journal: NeuroReport (2024): 648--656
Search for new biologically active compounds: in vitro studies of antitumor and antimicrobial activity of dirhodium (II, II) paddlewheel complexes
Authors: Mitrovi{\'c}, Marina and Djuki{\'c}, Maja B and Vuki{\'c}, Milena and Nikoli{\'c}, Ivana and Radovanovi{\'c}, Marko D and Lukovi{\'c}, Jovan and Filipovi{\'c}, Ignjat P and Mati{\'c}, Sanja and Markovi{\'c}, Tijana and Klisuri{\'c}, Olivera R and others,
Journal: Dalton Transactions (2024)
Antifungal activity of human antimicrobial peptides targeting apoptosis in Candida auris
Authors: Shaban, Siham and Patel, Mrudula and Ahmad, Aijaz
Journal: Journal of Medical Microbiology (2024): 001835
Amorphous CaCO3-bioreactor for tumor microenvironment regulation to reinforce tumor chemoimmunotherapy
Authors: Chen, Weiguo and Lu, Yishuang and Xu, Yize and Chen, Yujiang and Lin, Shuai and He, Xin and Zhang, Chunfeng and Yuan, Chunsu
Journal: Chemical Engineering Journal (2024): 151838

References


View all 6 references: Citation Explorer
Tissue plasminogen activator regulates Purkinje neuron development and survival
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
Application of a homogenous membrane potential assay to assess mitochondrial function
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
5,6-Dimethylxanthenone-4-acetic Acid (DMXAA) Activates Stimulator of Interferon Gene (STING)-dependent Innate Immune Pathways and Is Regulated by Mitochondrial Membrane Potential
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Human iPS-derived Cardiomyocytes for Cardiotoxicity Screening
Authors: , undefined
Journal: SBS Molecular Devices (2011)
iCell&reg; Cardiomyocytes: Assaying Mitochondrial Membrane Potential
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf
Analyzing Cellular Apoptosis Through Monitoring Mitochondrial Membrane Potential Changes with JC-10
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2