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LDS 651
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Application notes
LDS 651 is an analog of LDS 751. LDS 651 has almost identical intracellular staining pattern to LDS 751. LDS 651 is optimized to be excited by Violet Laser while LDS 751 is excited by Blue Laser at 488 nm. Both LDS 651 and LDS 751 are cell-permeant fluorescent dyes. Although both of LDS 651 and LDS 751 bind to DNA, they may be excluded from the nucleus and bind to other cellular structures such as the polarized membranes of mitochondria. Cautions need be taken when LDS 651 and LDS 751 are used for analyzing live cells and their fluorescence as being indicative of nuclear status. LDS 651 and LDS 751 might be used to separate red blood cells from nucleated cells.
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note Store the unused stock solution in small aliquots at -20 °C, protected from light.
LDS 651 stock solution
Prepare a 1 mM stock solution by adding the appropriate amount of DMSO.Note Store the unused stock solution in small aliquots at -20 °C, protected from light.
PREPARATION OF WORKING SOLUTION
LDS 651 working solution
Dilute the 1 mM LDS 651 stock solution to 1 to 10 µM LDS 651 working solution in the buffer of your choice.Note Prepare the working solution immediately before use.
Note The LDS 651 working solution should not be stored or reused.
Note The concentration of the LDS 651 working solution should be optimized for different cell types and conditions.
SAMPLE EXPERIMENTAL PROTOCOL
The following sample protocol is provided as a basic guide for the development of your own staining protocol. The concentrations of the reagents required for the optimal staining may vary depending on the density of cells (i.e. Leukocytes) and other materials in the sample.
- Add the LDS 651 working solution in samples.
- Incubate the samples at 37 °C for 5-10 minutes.
- Set a fluorescence threshold to detect cells stained positive with LDS-651, thus excluding erythrocytes and unbound single platelets from the display.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| LDS 751 *CAS 181885-68-7* | 561 | 712 |
References
View all 46 references: Citation Explorer
Quantifying permeabilization and activity recovery of Bacillus spores in adverse conditions for growth.
Authors: Trunet, C and Ngo, H and Coroller, L
Journal: Food microbiology (2019): 115-120
Authors: Trunet, C and Ngo, H and Coroller, L
Journal: Food microbiology (2019): 115-120
Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase.
Authors: Grekov, Igor and Pombinho, António R and Kobets, Tatyana and Bartůněk, Petr and Lipoldová, Marie
Journal: BioMed research international (2017): 1309485
Authors: Grekov, Igor and Pombinho, António R and Kobets, Tatyana and Bartůněk, Petr and Lipoldová, Marie
Journal: BioMed research international (2017): 1309485
Lipid bilayer properties control membrane partitioning, binding, and transport of p-glycoprotein substrates.
Authors: Clay, Adam T and Sharom, Frances J
Journal: Biochemistry (2013): 343-54
Authors: Clay, Adam T and Sharom, Frances J
Journal: Biochemistry (2013): 343-54
Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Archives of biochemistry and biophysics (2009): 17-28
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Archives of biochemistry and biophysics (2009): 17-28
Flow cytometric assessment of homeostatic aging of reticulocytes in rats.
Authors: Wiczling, Paweł and Krzyzanski, Wojciech
Journal: Experimental hematology (2008): 119-27
Authors: Wiczling, Paweł and Krzyzanski, Wojciech
Journal: Experimental hematology (2008): 119-27