LDS 651

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	473.40
Solvent	DMSO

Spectral properties

Excitation (nm)	557
Emission (nm)	630

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

473.40

Excitation (nm)

557

Emission (nm)

630

LDS 651 is an analog of LDS 751. LDS 651 has almost identical intracellular staining pattern to LDS 751. LDS 651 is optimized to be excited by Violet Laser while LDS 751 is excited by Blue Laser at 488 nm. Both LDS 651 and LDS 751 are cell-permeant fluorescent dyes. Although both of LDS 651 and LDS 751 bind to DNA, they may be excluded from the nucleus and bind to other cellular structures such as the polarized membranes of mitochondria. Cautions need be taken when LDS 651 and LDS 751 are used for analyzing live cells and their fluorescence as being indicative of nuclear status. LDS 651 and LDS 751 might be used to separate red blood cells from nucleated cells.

Platform

Flow cytometer

Excitation	488 nm laser
Emission	695/40 nm filter
Instrument specification(s)	PerCP channel

Example protocol

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

LDS 651 stock solution

Prepare a 1 mM stock solution by adding the appropriate amount of DMSO.
Note Store the unused stock solution in small aliquots at -20 °C, protected from light.

PREPARATION OF WORKING SOLUTION

LDS 651 working solution

Dilute the 1 mM LDS 651 stock solution to 1 to 10 µM LDS 651 working solution in the buffer of your choice.
Note     Prepare the working solution immediately before use.
Note     The LDS 651 working solution should not be stored or reused.
Note     The concentration of the LDS 651 working solution should be optimized for different cell types and conditions.

SAMPLE EXPERIMENTAL PROTOCOL

The following sample protocol is provided as a basic guide for the development of your own staining protocol. The concentrations of the reagents required for the optimal staining may vary depending on the density of cells (i.e. Leukocytes) and other materials in the sample.

Add the LDS 651 working solution in samples.
Incubate the samples at 37 °C for 5-10 minutes.
Set a fluorescence threshold to detect cells stained positive with LDS-651, thus excluding erythrocytes and unbound single platelets from the display.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LDS 651 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.238 µL	1.056 mL	2.112 mL	10.562 mL	21.124 mL
5 mM	42.248 µL	211.238 µL	422.476 µL	2.112 mL	4.225 mL
10 mM	21.124 µL	105.619 µL	211.238 µL	1.056 mL	2.112 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	557
Emission (nm)	630

Product Family

Name	Excitation (nm)	Emission (nm)
LDS 751 CAS 181885-68-7	561	712

Images

Figure 1. Flow cytometric analysis with LDS 651. Jurkat cells were stained with LDS 651 as per the protocol and response was measured with NovoCyte flow cytometer using PerCP channel.

References

View all 46 references: Citation Explorer

Quantifying permeabilization and activity recovery of Bacillus spores in adverse conditions for growth.
Authors: Trunet, C and Ngo, H and Coroller, L
Journal: Food microbiology (2019): 115-120

Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase.
Authors: Grekov, Igor and Pombinho, António R and Kobets, Tatyana and Bartůněk, Petr and Lipoldová, Marie
Journal: BioMed research international (2017): 1309485

Lipid bilayer properties control membrane partitioning, binding, and transport of p-glycoprotein substrates.
Authors: Clay, Adam T and Sharom, Frances J
Journal: Biochemistry (2013): 343-54

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Archives of biochemistry and biophysics (2009): 17-28

Flow cytometric assessment of homeostatic aging of reticulocytes in rats.
Authors: Wiczling, Paweł and Krzyzanski, Wojciech
Journal: Experimental hematology (2008): 119-27

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule.
Authors: Guo, Xunmin and Wang, Sufan and Xia, Andong and Su, Hongmei
Journal: The journal of physical chemistry. A (2007): 5800-5

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry.
Authors: Maes, Melissa L and Davidson, Lisa B and McDonagh, Paul F and Ritter, Leslie S
Journal: Journal of immunological methods (2007): 79-86

A novel flow cytometric assay of human whole blood neutrophil and monocyte CD11b levels: upregulation by chemokines is related to receptor expression, comparison with neutrophil shape change, and effects of a chemokine receptor (CXCR2) antagonist.
Authors: Nicholson, Grant C and Tennant, Rachel C and Carpenter, Donald C and Sarau, Henry M and Kon, Onn Min and Barnes, Peter J and Salmon, Michael and Vessey, Rupert S and Tal-Singer, Ruth and Hansel, Trevor T
Journal: Pulmonary pharmacology & therapeutics (2007): 52-9

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Biochemistry (2005): 14020-9

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry.
Authors: Fritsch, M and Starruss, J and Loesche, A and Mueller, S and Bley, Th
Journal: Biotechnology and bioengineering (2005): 635-42