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LDS 751 *CAS 181885-68-7*

Chemical structure for LDS 751 *CAS 181885-68-7*
Chemical structure for LDS 751 *CAS 181885-68-7*
Ordering information
Price ()
Catalog Number17561
Unit Size
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Physical properties
Molecular weight471.98
SolventDMSO
Spectral properties
Excitation (nm)561
Emission (nm)712
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
181885-68-7
Molecular weight
471.98
Excitation (nm)
561
Emission (nm)
712
LDS 751 is a fluorescent cell-permeant nucleic acid stain that can be well excited with 488 nm laser line although it has a peak excitation at ~543 nm on dsDNA. It might be an alternative to DRAQ 5™, useful in multicolor analyses due to its long wavelength emission maximum (~712 nm). Upon binding to dsDNA of LDS 751 has ~20-fold fluorescence enhancement. It has been reported that LDS-751 is excluded from the nucleus and binds the polarized membranes of mitochondria. Thus cautions need be taken when LDS 751 is used for analyzing live cells and LDS-751 fluorescence as being indicative of nuclear status. Thiazole orange (an RNA dye) and LDS-751 (a DNA dye) composition has been used to separate reticulocytes from other cell populations. LDS 751 has been also used to separate red blood cells from nucleated cells.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LDS 751 *CAS 181885-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM211.873 µL1.059 mL2.119 mL10.594 mL21.187 mL
5 mM42.375 µL211.873 µL423.747 µL2.119 mL4.237 mL
10 mM21.187 µL105.937 µL211.873 µL1.059 mL2.119 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)561
Emission (nm)712

Product family


NameExcitation (nm)Emission (nm)
LDS 651557630

References


View all 29 references: Citation Explorer
Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17
Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256
Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49
Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119
Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79
Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800
Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020
Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030
Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635
Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251