LDS 751 *CAS 181885-68-7*
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 471.98 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 561 |
| Emission (nm) | 712 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
| Overview |  SDSProtocol |
CAS 181885-68-7 | Molecular weight 471.98 | Excitation (nm) 561 | Emission (nm) 712 |
LDS 751 is a fluorescent cell-permeant nucleic acid stain that can be well excited with 488 nm laser line although it has a peak excitation at ~543 nm on dsDNA. It might be an alternative to DRAQ 5™, useful in multicolor analyses due to its long wavelength emission maximum (~712 nm). Upon binding to dsDNA of LDS 751 has ~20-fold fluorescence enhancement. It has been reported that LDS-751 is excluded from the nucleus and binds the polarized membranes of mitochondria. Thus cautions need be taken when LDS 751 is used for analyzing live cells and LDS-751 fluorescence as being indicative of nuclear status. Thiazole orange (an RNA dye) and LDS-751 (a DNA dye) composition has been used to separate reticulocytes from other cell populations. LDS 751 has been also used to separate red blood cells from nucleated cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of LDS 751 *CAS 181885-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 211.873 µL | 1.059 mL | 2.119 mL | 10.594 mL | 21.187 mL |
| 5 mM | 42.375 µL | 211.873 µL | 423.747 µL | 2.119 mL | 4.237 mL |
| 10 mM | 21.187 µL | 105.937 µL | 211.873 µL | 1.059 mL | 2.119 mL |
Molarity calculator
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Images
Figure 1. Chemical structure for LDS 751 *CAS 181885-68-7*
References
View all 29 references: Citation Explorer
Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17
Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256
Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49
Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119
Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79
Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800
Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020
Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030
Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635
Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251