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Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*
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Leu-AMC is a fluorogenic substrate for leucine aminopeptidase. Upon the hydrolysis by proteases, Leu-AMC releases the strongly fluorescent AMC fluorophore.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 307.882 µL | 1.539 mL | 3.079 mL | 15.394 mL | 30.788 mL |
| 5 mM | 61.576 µL | 307.882 µL | 615.764 µL | 3.079 mL | 6.158 mL |
| 10 mM | 30.788 µL | 153.941 µL | 307.882 µL | 1.539 mL | 3.079 mL |
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References
View all 39 references: Citation Explorer
Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target
Authors: Munkhjargal T, Ishizaki T, Guswanto A, Takemae H, Yokoyama N, Igarashi I.
Journal: Vet Parasitol (2016): 14
Authors: Munkhjargal T, Ishizaki T, Guswanto A, Takemae H, Yokoyama N, Igarashi I.
Journal: Vet Parasitol (2016): 14
Secretory Expression, Purification, Characterization, and Application of an Aspergillus oryzae Prolyl Aminopeptidase in Bacillus subtilis
Authors: Wang KD, Wang KH, Zhou ND, Tian YP.
Journal: Appl Biochem Biotechnol. (2016)
Authors: Wang KD, Wang KH, Zhou ND, Tian YP.
Journal: Appl Biochem Biotechnol. (2016)
Inhibition of leucine aminopeptidase 3 suppresses invasion of ovarian cancer cells through down-regulation of fascin and MMP-2/9
Authors: Wang X, Shi L, Deng Y, Qu M, Mao S, Xu L, Xu W, Fang C.
Journal: Eur J Pharmacol (2015): 116
Authors: Wang X, Shi L, Deng Y, Qu M, Mao S, Xu L, Xu W, Fang C.
Journal: Eur J Pharmacol (2015): 116
Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii
Authors: Lee YR, Na BK, Moon EK, Song SM, Joo SY, Kong HH, Goo YK, Chung DI, Hong Y.
Journal: PLoS One (2015): e0129884
Authors: Lee YR, Na BK, Moon EK, Song SM, Joo SY, Kong HH, Goo YK, Chung DI, Hong Y.
Journal: PLoS One (2015): e0129884
Molecular Characterization of Babesia bovis M17 Leucine Aminopeptidase and Inhibition of Babesia Growth by Bestatin
Authors: Aboge GO, Cao S, Terkawi MA, Masatani T, Goo Y, AbouLaila M, Nishikawa Y, Igarashi I, Suzuki H, Xuan X.
Journal: J Parasitol (2015): 536
Authors: Aboge GO, Cao S, Terkawi MA, Masatani T, Goo Y, AbouLaila M, Nishikawa Y, Igarashi I, Suzuki H, Xuan X.
Journal: J Parasitol (2015): 536