logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Live or Dead™ Fixable Dead Cell Staining Kit
Blue Fluorescence
Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for long term microscopic examination. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The fluorescent dye used in the kit is quite photostable so that the images can be repeatedly examined. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.
Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22600). Jurkat cells were treated and stained with Stain It™ Blue . The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22600). Jurkat cells were treated and stained with Stain It™ Blue . The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22600200 Tests
Price
 
Spectral properties
Excitation (nm)346
Emission (nm)434
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation355 nm or 405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel
Fluorescence microscope
Excitation353 nm
Emission442 nm
Recommended plateBlack wall/clear bottom
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025