Mag-Fluo-4 AM

The cell-permeant Mag-Fluo-4 AM is an analog of Fluo-4 AM with a Kd for Mg ion of 4.7 mM and a Kd for Ca ion of 22 µM, making it useful as an intracellular Mg ion indicator as well as a low-affinity Ca ion indicator. This low-affinity fluorescent Ca ion indicator has been used to accurately track the kinetics of the spatially averaged free Ca ion transient in skeletal muscle. Mag-fluo-4 yields reliable kinetic information about the spatially averaged free Ca ion transient in skeletal muscle.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20401	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Mag-Fluo-4 AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.302 µL	611.509 µL	1.223 mL	6.115 mL	12.23 mL
5 mM	24.46 µL	122.302 µL	244.603 µL	1.223 mL	2.446 mL
10 mM	12.23 µL	61.151 µL	122.302 µL	611.509 µL	1.223 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	817.65 g/mol	=	mL	x	mM	=	mmol

Citations

View all 16 citations: Citation Explorer

Cynaropicrin Increases [Ca2+] i and Ciliary Beat Frequency in Human Airway Epithelial Cells by Inhibiting SERCA

Authors:

Todo, Nobuhisa and Hosogi, Shigekuni and Nakamura, Seikou and Noriyama, Kouta and Tamiya, Nobuyo and Toda, Yuki and Shigeta, Masaki and Takayama, Koichi and Ashihara, Eishi

Journal:

Biological and Pharmaceutical Bulletin (2024): 2119--2126

TRPV1 alleviates APOE4-dependent microglial antigen presentation and T cell infiltration in Alzheimer's disease

Authors:

Lu, Jia and Wu, Kexin and Sha, Xudong and Lin, Jiayuan and Chen, Hongzhuan and Yu, Zhihua

Journal:

Translational Neurodegeneration (2024): 52

African Swine Fever Virus Modulates the Endoplasmic Reticulum Stress-ATF6-Calcium Axis to Facilitate Viral Replication: ASFV modulates ER stress-ATF6-Ca2+ axis for replication

Authors:

Wang, Yanjin and Li, Jiaqi and Cao, Hongwei and Li, Lian-Feng and Dai, Jingwen and Cao, Mengxiang and Deng, Hao and Zhong, Dailang and Luo, Yuzi and Li, Yongfeng and others,

Journal:

Emerging Microbes \& Infections (2024): 2399945

Sulfated Laminarin Polysaccharides Reduce the Adhesion of Nano-COM Crystals to Renal Epithelial Cells by Inhibiting Oxidative and Endoplasmic Reticulum Stress

Authors:

He, Tian-Qu and Wang, Zhi and Li, Chuang-Ye and Zhao, Yao-Wang and Tong, Xin-Yi and Liu, Jing-Hong and Ouyang, Jian-Ming

Journal:

Pharmaceuticals (2024): 805

Mitochondrial-dependent oxidative phosphorylation is key for postnatal metabolic adaptation of alveolar macrophages in the lung

Authors:

Zhang, Jun and Peng, Yu and Song, Haosen and Liu, Siqi and Li, Chuanwei and Zhang, Yi and Shi, Xiaowei and Guo, Huifang and Xu, Yingping

Journal:

International Immunopharmacology (2024): 112012

References

View all 25 references: Citation Explorer

Real-time intra-store confocal Ca2+ imaging in isolated mouse cardiomyocytes

Authors:

Fern, undefined and ez-Tenorio M, Niggli E.

Journal:

Cell Calcium. (2016)

Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead

Authors:

Wang H, Wang ZK, Jiao P, Zhou XP, Yang DB, Wang ZY, Wang L.

Journal:

Toxicology (2015): 137

Role of Mitofusin-2 in mitochondrial localization and calcium uptake in skeletal muscle

Authors:

Ainbinder A, Boncompagni S, Protasi F, Dirksen RT.

Journal:

Cell Calcium (2015): 14

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach

Authors:

Calderon JC, Bolanos P, Caputo C.

Journal:

J Muscle Res Cell Motil (2014): 279

TRPC1 is involved in Ca(2)(+) influx and cytotoxicity following Pb(2)(+) exposure in human embryonic kidney cells

Authors:

Zhang H, Li W, Xue Y, Zou F.

Journal:

Toxicol Lett (2014): 52

Physical properties

Dissociation constant (K_d, nM)	22000
Molecular weight	817.65
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	495
Emission (nm)	528
Quantum yield	0.16 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025