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Mag-Fluo-4 AM
The cell-permeant Mag-Fluo-4 AM is an analog of Fluo-4 AM with a Kd for Mg ion of 4.7 mM and a Kd for Ca ion of 22 µM, making it useful as an intracellular Mg ion indicator as well as a low-affinity Ca ion indicator. This low-affinity fluorescent Ca ion indicator has been used to accurately track the kinetics of the spatially averaged free Ca ion transient in skeletal muscle. Mag-fluo-4 yields reliable kinetic information about the spatially averaged free Ca ion transient in skeletal muscle.
Chemical structure for Mag-Fluo-4 AM
Chemical structure for Mag-Fluo-4 AM
CatalogSize
Price
Quantity
2040110x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)22000
Molecular weight817.65
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)495
Emission (nm)528
Quantum yield
0.16
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025