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mFluor™ 510-VAD-FMK
FAM-VAD is a green fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. The probe has the spectral properties similar to BD Horizon™ V500, thus the filter set of BD Horizon™ V500 can be conveniently used with a flow cytometer or microscope. This probe is particularly designed for the multicolor detection of caspases with a violet laser-equipped flow cytomer.
Chemical structure for mFluor™ 510-VAD-FMK
Chemical structure for mFluor™ 510-VAD-FMK
CatalogSize
Price
Quantity
1347625 Tests
Price
 
Physical properties

Molecular weight708.19
SolventDMSO
Spectral properties

Absorbance (nm)410
Correction factor (260 nm)0.464
Correction factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)
25000
1
Excitation (nm)412
Emission (nm)505
Quantum yield
0.86
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 3, 2025