Name: mFluor™ 510-VAD-FMK
Brand: AAT Bioquest
SKU: 13476
Price: 115 USD
Availability: InStock

mFluor™ 510-VAD-FMK

FAM-VAD is a green fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. The probe has the spectral properties similar to BD Horizon™ V500, thus the filter set of BD Horizon™ V500 can be conveniently used with a flow cytometer or microscope. This probe is particularly designed for the multicolor detection of caspases with a violet laser-equipped flow cytomer.

Protocol

SDS

Catalog	Size	Price	Quantity
13476	25 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ 510-VAD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	141.205 µL	706.025 µL	1.412 mL	7.06 mL	14.121 mL
5 mM	28.241 µL	141.205 µL	282.41 µL	1.412 mL	2.824 mL
10 mM	14.121 µL	70.603 µL	141.205 µL	706.025 µL	1.412 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	708.19 g/mol	=	mL	x	mM	=	mmol

References

View all 26 references: Citation Explorer

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties

Authors:

Lawrence CP, Chow SC.

Journal:

Toxicol Appl Pharmacol. (2012)

Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice

Authors:

Li YY, Yan CL.

Journal:

Zhonghua Yi Xue Za Zhi (2012): 1992

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death

Authors:

Meslin B, Beavogui AH, Fasel N, Picot S.

Journal:

PLoS One (2011): e23867

Physical properties

Molecular weight	708.19
Solvent	DMSO

Spectral properties

Absorbance (nm)	410
Correction factor (260 nm)	0.464
Correction factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Enzyme Probes & Assay Kits

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Page updated on October 29, 2025