mFluor™ Blue 570 Anti-human CD73 Antibody AD2

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Absorbance (nm)	553
Correction Factor (260 nm)	0.228
Correction Factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	505
Emission (nm)	564

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

NT5E; Ecto-5'-nucleotidase

Clone

AD2

Host

mouse

Isotype

Mouse IgG1 kappa

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Absorbance (nm)

553

Correction Factor (260 nm)

0.228

Correction Factor (280 nm)

0.179

Extinction coefficient (cm ^-1 M ^-1)

120000¹

Excitation (nm)

505

Emission (nm)

564

AD2 is an anti-human monoclonal antibody that is specific for the CD73 antigen. CD73 (sometimes called NT5E or Ecto-5'-nucleotidase) is a 70 kD transmembrane protein that is found on the surface of cells such as epithelial cells, endothelial cells and stem cells. CD73 is associated with a variety of biologically interesting macromolecules/ligands, for instance, AMP. CD73 is a moderately popular antibody target, with over 11000 publications in the last decade. CD73 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells. This antibody was purified through affinity chromatography and conjugated to mFluor™ Blue 570 (ex/em = 553/565 nm). It is compatible with the 561 nm laser and 585/29 nm bandpass filter (for example, as in the BD FACSJazz™).

Spectrum

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Spectral properties

Absorbance (nm)	553
Correction Factor (260 nm)	0.228
Correction Factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	505
Emission (nm)	564

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd73 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780