Name: mFluor™ Green 620 Anti-human CD47 Antibody *B6.H12*
Brand: AAT Bioquest
SKU: 104720U0
Availability: InStock

mFluor™ Green 620 Anti-human CD47 Antibody

B6.H12

B6.H12 is an anti-human monoclonal antibody that is specific for the CD47 antigen. CD47 (sometimes called MER6, Integrin associated protein or integrin-associated protein) is a 42 - 52 kD multi-pass membrane protein that is located on the surface of cells like T cells, erythrocytes, epithelial cells and endothelial cells. CD47 acts in essential cellular pathways, for instance, the negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis and integrin-mediated signaling pathway. Also, in some organisms, it is involved in the positive regulation of stress fiber assembly, is a promoter of T cell activation and positively regulates phagocytosis. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Green 620 (ex/em = 525/623 nm). It is compatible with the 532 nm laser and 620/15 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Datasheet

COA

Catalog	Size	Price	Quantity
104720U0	100 tests	Price
104720U1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD47 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	gp42, IAP, neurophilin, MER6, Integrin associated protein
Clone	B6.H12
Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	525
Correction factor (260 nm)	0.895
Correction factor (280 nm)	0.569
Extinction coefficient (cm ^-1 M ^-1)	50000 ¹
Excitation (nm)	525
Emission (nm)	623

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 1, 2026