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mFluor™ UV 460 Biotin Conjugate

mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence.
mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence.
Ordering information
Price ()
Catalog Number3112
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight538.57
SolventDMSO
Spectral properties
Absorbance (nm)364
Correction Factor (260 nm)0.35
Correction Factor (280 nm)0.134
Extinction coefficient (cm -1 M -1)150001
Excitation (nm)358
Emission (nm)456
Quantum yield0.861
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
538.57
Absorbance (nm)
364
Correction Factor (260 nm)
0.35
Correction Factor (280 nm)
0.134
Extinction coefficient (cm -1 M -1)
150001
Excitation (nm)
358
Emission (nm)
456
Quantum yield
0.861
mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ UV 460 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UV 460 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM185.677 µL928.384 µL1.857 mL9.284 mL18.568 mL
5 mM37.135 µL185.677 µL371.354 µL1.857 mL3.714 mL
10 mM18.568 µL92.838 µL185.677 µL928.384 µL1.857 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)364
Correction Factor (260 nm)0.35
Correction Factor (280 nm)0.134
Extinction coefficient (cm -1 M -1)150001
Excitation (nm)358
Emission (nm)456
Quantum yield0.861

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 Biotin Conjugate3513873000010.9410.0990.138

References


View all 14 references: Citation Explorer
Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Temperature-Responsive Hydrogel-Coated Gold Nanoshells.
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization.
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Genetic engineering, expression, and activity of a chimeric monoclonal antibody-avidin fusion protein for receptor-mediated delivery of biotinylated drugs in humans.
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin.
Authors: Wu, Yang and Simons, Peter C and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Analytical biochemistry (2005): 221-8
Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry.
Authors: Wu, Yang and Zwartz, Gordon and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 37-44
Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys.
Authors: Van Loock, Marnix and Verminnen, Kristel and Messmer, Trudy O and Volckaert, Guido and Goddeeris, Bruno M and Vanrompay, Daisy
Journal: BMC infectious diseases (2005): 76
On-line continuous-flow, multi-protein biochemical assays for the characterization of bioaffinity compounds using electrospray quadrupole time-of-flight mass spectrometry.
Authors: Derks, R J E and Hogenboom, A C and van der Zwan, G and Irth, H
Journal: Analytical chemistry (2003): 3376-84
Optical measurement of transverse molecular diffusion in a microchannel.
Authors: Kamholz, A E and Schilling, E A and Yager, P
Journal: Biophysical journal (2001): 1967-72
Organelle pH studies using targeted avidin and fluorescein-biotin.
Authors: Wu, M M and Llopis, J and Adams, S and McCaffery, J M and Kulomaa, M S and Machen, T E and Moore, H P and Tsien, R Y
Journal: Chemistry & biology (2000): 197-209