mFluor™ UV 460 Biotin Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 538.57 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 364 |
| Correction Factor (260 nm) | 0.35 |
| Correction Factor (280 nm) | 0.134 |
| Extinction coefficient (cm -1 M -1) | 150001 |
| Excitation (nm) | 358 |
| Emission (nm) | 456 |
| Quantum yield | 0.861 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 538.57 | Absorbance (nm) 364 | Correction Factor (260 nm) 0.35 | Correction Factor (280 nm) 0.134 | Extinction coefficient (cm -1 M -1) 150001 | Excitation (nm) 358 | Emission (nm) 456 | Quantum yield 0.861 |
mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ UV 460 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UV 460 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 185.677 µL | 928.384 µL | 1.857 mL | 9.284 mL | 18.568 mL |
| 5 mM | 37.135 µL | 185.677 µL | 371.354 µL | 1.857 mL | 3.714 mL |
| 10 mM | 18.568 µL | 92.838 µL | 185.677 µL | 928.384 µL | 1.857 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 364 |
| Correction Factor (260 nm) | 0.35 |
| Correction Factor (280 nm) | 0.134 |
| Extinction coefficient (cm -1 M -1) | 150001 |
| Excitation (nm) | 358 |
| Emission (nm) | 456 |
| Quantum yield | 0.861 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ UV 375 Biotin Conjugate | 351 | 387 | 300001 | 0.941 | 0.099 | 0.138 |
Images
Figure 1. mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence.
References
View all 14 references: Citation Explorer
Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Temperature-Responsive Hydrogel-Coated Gold Nanoshells.
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization.
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Genetic engineering, expression, and activity of a chimeric monoclonal antibody-avidin fusion protein for receptor-mediated delivery of biotinylated drugs in humans.
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin.
Authors: Wu, Yang and Simons, Peter C and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Analytical biochemistry (2005): 221-8
Authors: Wu, Yang and Simons, Peter C and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Analytical biochemistry (2005): 221-8
Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry.
Authors: Wu, Yang and Zwartz, Gordon and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 37-44
Authors: Wu, Yang and Zwartz, Gordon and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 37-44
Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys.
Authors: Van Loock, Marnix and Verminnen, Kristel and Messmer, Trudy O and Volckaert, Guido and Goddeeris, Bruno M and Vanrompay, Daisy
Journal: BMC infectious diseases (2005): 76
Authors: Van Loock, Marnix and Verminnen, Kristel and Messmer, Trudy O and Volckaert, Guido and Goddeeris, Bruno M and Vanrompay, Daisy
Journal: BMC infectious diseases (2005): 76
On-line continuous-flow, multi-protein biochemical assays for the characterization of bioaffinity compounds using electrospray quadrupole time-of-flight mass spectrometry.
Authors: Derks, R J E and Hogenboom, A C and van der Zwan, G and Irth, H
Journal: Analytical chemistry (2003): 3376-84
Authors: Derks, R J E and Hogenboom, A C and van der Zwan, G and Irth, H
Journal: Analytical chemistry (2003): 3376-84
Optical measurement of transverse molecular diffusion in a microchannel.
Authors: Kamholz, A E and Schilling, E A and Yager, P
Journal: Biophysical journal (2001): 1967-72
Authors: Kamholz, A E and Schilling, E A and Yager, P
Journal: Biophysical journal (2001): 1967-72
Organelle pH studies using targeted avidin and fluorescein-biotin.
Authors: Wu, M M and Llopis, J and Adams, S and McCaffery, J M and Kulomaa, M S and Machen, T E and Moore, H P and Tsien, R Y
Journal: Chemistry & biology (2000): 197-209
Authors: Wu, M M and Llopis, J and Adams, S and McCaffery, J M and Kulomaa, M S and Machen, T E and Moore, H P and Tsien, R Y
Journal: Chemistry & biology (2000): 197-209