mFluor™ UV420-streptavidin conjugate
Ordering information
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Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | Water |
Spectral properties
Absorbance (nm) | 352 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 353 |
Emission (nm) | 421 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Absorbance (nm) 352 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 353 | Emission (nm) 421 |
mFluor™ UV420 streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ UV420 covalently attached. mFluor™ UV420 provides a unique color for fluorescence imaging and flow cytometric applications. It is used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. AAT Bioquest offers the largest collection of streptavidin conjugates. They are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. The affinity between streptavidin and biotin might be the strongest non-covalent interactions known in biological interactions. Streptavidin, a homotetrameric protein, exhibits an extraordinarily high affinity for biotin. Each streptavidin monomer can bind one biotin molecule, allowing a streptavidin protein to maximally bind four biotins. The streptavidin-biotin interaction is highly specific and remains robust under a wide range of conditions.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 352 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 353 |
Emission (nm) | 421 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ UV455-streptavidin conjugate | 357 | 461 | 200001 | 0.421 | 0.651 | 0.406 |
References
View all 11 references: Citation Explorer
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Authors: Jin, Yu and Bae, Jaeyoung and Kim, Tehee Yurie and Hwang, Hyeseung and Kim, Taesoo and Yu, Myungheon and Oh, Hyesoo and Hashiya, Kaori and Bando, Toshikazu and Sugiyama, Hiroshi and Jo, Kyubong
Journal: Analytical chemistry (2022): 16927-16935
Authors: Jin, Yu and Bae, Jaeyoung and Kim, Tehee Yurie and Hwang, Hyeseung and Kim, Taesoo and Yu, Myungheon and Oh, Hyesoo and Hashiya, Kaori and Bando, Toshikazu and Sugiyama, Hiroshi and Jo, Kyubong
Journal: Analytical chemistry (2022): 16927-16935
Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices.
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8
Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array.
Authors: Jacob, Francis and Goldstein, Darlene R and Bovin, Nicolai V and Pochechueva, Tatiana and Spengler, Marianne and Caduff, Rosemarie and Fink, Daniel and Vuskovic, Marko I and Huflejt, Margaret E and Heinzelmann-Schwarz, Viola
Journal: International journal of cancer (2012): 138-46
Authors: Jacob, Francis and Goldstein, Darlene R and Bovin, Nicolai V and Pochechueva, Tatiana and Spengler, Marianne and Caduff, Rosemarie and Fink, Daniel and Vuskovic, Marko I and Huflejt, Margaret E and Heinzelmann-Schwarz, Viola
Journal: International journal of cancer (2012): 138-46
Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophan residues in a protein via Főrster-resonance-energy-transfer.
Authors: Xie, Yanling and Yang, Xiaolan and Pu, Jun and Zhao, Yunsheng and Zhang, Ying and Xie, Guoming and Zheng, Jun and Yuan, Huidong and Liao, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2010): 869-76
Authors: Xie, Yanling and Yang, Xiaolan and Pu, Jun and Zhao, Yunsheng and Zhang, Ying and Xie, Guoming and Zheng, Jun and Yuan, Huidong and Liao, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2010): 869-76
[The biotin-thyroxin conjugate as a bifunctional ligand of binding proteins].
Authors: Novakovskiĭ, M E and Vashkevich, I I and Sviridov, O V
Journal: Bioorganicheskaia khimiia (2009): 178-91
Authors: Novakovskiĭ, M E and Vashkevich, I I and Sviridov, O V
Journal: Bioorganicheskaia khimiia (2009): 178-91
Zinc(II)-coordinated oligotyrosine: a new class of cell penetrating peptide.
Authors: Johnson, James R and Jiang, Hua and Smith, Bradley D
Journal: Bioconjugate chemistry (2008): 1033-9
Authors: Johnson, James R and Jiang, Hua and Smith, Bradley D
Journal: Bioconjugate chemistry (2008): 1033-9
Homogeneous non-competitive bioaffinity assay based on fluorescence resonance energy transfer.
Authors: Kokko, Tiina and Kokko, Leena and Soukka, Tero and Lövgren, Timo
Journal: Analytica chimica acta (2007): 120-5
Authors: Kokko, Tiina and Kokko, Leena and Soukka, Tero and Lövgren, Timo
Journal: Analytica chimica acta (2007): 120-5
A cGMP-dependent protein kinase assay for high throughput screening based on time-resolved fluorescence resonance energy transfer.
Authors: Bader, B and Butt, E and Palmetshofer, A and Walter, U and Jarchau, T and Drueckes, P
Journal: Journal of biomolecular screening (2001): 255-64
Authors: Bader, B and Butt, E and Palmetshofer, A and Walter, U and Jarchau, T and Drueckes, P
Journal: Journal of biomolecular screening (2001): 255-64
cFos immunoreactivity is enhanced with biotin amplification.
Authors: Berghorn, K A and Bonnett, J H and Hoffman, G E
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1994): 1635-42
Authors: Berghorn, K A and Bonnett, J H and Hoffman, G E
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1994): 1635-42
Biotin binding changes the conformation and decreases tryptophan accessibility of streptavidin.
Authors: Kurzban, G P and Gitlin, G and Bayer, E A and Wilchek, M and Horowitz, P M
Journal: Journal of protein chemistry (1990): 673-82
Authors: Kurzban, G P and Gitlin, G and Bayer, E A and Wilchek, M and Horowitz, P M
Journal: Journal of protein chemistry (1990): 673-82