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mFluor™ UV455-streptavidin conjugate
mFluor™ UV455 streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ UV 455 fluorescent dyes. Its high affinity for biotinylated conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ UV455 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by an ultraviolet laser, mFluor™ UV455 exhibits a bright and photostable emission of blue fluorescence at 461 nm. mFluor™ UV455 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.
product image
product image
CatalogSize
Price
Quantity
47200100 ug
Price
472011 mg
Price
 
Physical properties

Molecular weight~52 kDa
SolventWater
Spectral properties

Absorbance (nm)359
Correction factor (260 nm)0.651
Correction factor (280 nm)0.406
Extinction coefficient (cm -1 M -1)
20000
1
Excitation (nm)357
Emission (nm)461
Quantum yield
0.42
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025