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mFluor™ UV455-streptavidin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | ~52 kDa |
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 359 |
| Correction Factor (260 nm) | 0.651 |
| Correction Factor (280 nm) | 0.406 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 357 |
| Emission (nm) | 461 |
| Quantum yield | 0.421 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDS |
Molecular weight ~52 kDa | Absorbance (nm) 359 | Correction Factor (260 nm) 0.651 | Correction Factor (280 nm) 0.406 | Extinction coefficient (cm -1 M -1) 200001 | Excitation (nm) 357 | Emission (nm) 461 | Quantum yield 0.421 |
mFluor™ UV455 streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ UV 455 fluorescent dyes. Its high affinity for biotinylated conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ UV455 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by an ultraviolet laser, mFluor™ UV455 exhibits a bright and photostable emission of blue fluorescence at 461 nm. mFluor™ UV455 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 359 |
| Correction Factor (260 nm) | 0.651 |
| Correction Factor (280 nm) | 0.406 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 357 |
| Emission (nm) | 461 |
| Quantum yield | 0.421 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| mFluor™ UV420-streptavidin conjugate | 353 | 421 | 750001 |
References
View all 12 references: Citation Explorer
Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore.
Authors: Lee, Molly M and Peterson, Blake R
Journal: ACS omega (2016): 1266-1276
Authors: Lee, Molly M and Peterson, Blake R
Journal: ACS omega (2016): 1266-1276
Discrimination between streptavidin and avidin with fluorescent affinity-based probes.
Authors: Sun, Qian and Tian, Haiyu and Qu, Haoran and Sun, Deheng and Chen, Zhuo and Duan, Liping and Zhang, Weibing and Qian, Junhong
Journal: The Analyst (2015): 4648-53
Authors: Sun, Qian and Tian, Haiyu and Qu, Haoran and Sun, Deheng and Chen, Zhuo and Duan, Liping and Zhang, Weibing and Qian, Junhong
Journal: The Analyst (2015): 4648-53
Fluorescence-based screening assays for the NAD⁺-dependent histone deacetylase smSirt2 from Schistosoma mansoni.
Authors: Schiedel, Matthias and Marek, Martin and Lancelot, Julien and Karaman, Berin and Almlöf, Ingrid and Schultz, Johan and Sippl, Wolfgang and Pierce, Raymond J and Romier, Christophe and Jung, Manfred
Journal: Journal of biomolecular screening (2015): 112-21
Authors: Schiedel, Matthias and Marek, Martin and Lancelot, Julien and Karaman, Berin and Almlöf, Ingrid and Schultz, Johan and Sippl, Wolfgang and Pierce, Raymond J and Romier, Christophe and Jung, Manfred
Journal: Journal of biomolecular screening (2015): 112-21
Discovery of coumarin derivatives as fluorescence acceptors for intrinsic fluorescence resonance energy transfer of proteins.
Authors: Kim, Ju Hwan and Sumranjit, Jitapa and Kang, Hyo Jin and Chung, Sang J
Journal: Molecular bioSystems (2014): 30-3
Authors: Kim, Ju Hwan and Sumranjit, Jitapa and Kang, Hyo Jin and Chung, Sang J
Journal: Molecular bioSystems (2014): 30-3
Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells.
Authors: Sun, Qian and Qian, Junhong and Tian, Haiyu and Duan, Liping and Zhang, Weibing
Journal: Chemical communications (Cambridge, England) (2014): 8518-21
Authors: Sun, Qian and Qian, Junhong and Tian, Haiyu and Duan, Liping and Zhang, Weibing
Journal: Chemical communications (Cambridge, England) (2014): 8518-21
A novel small Odorranalectin-bearing cubosomes: preparation, brain delivery and pharmacodynamic study on amyloid-β₂₅₋₃₅-treated rats following intranasal administration.
Authors: Wu, Hongbing and Li, Jianxu and Zhang, Qizhi and Yan, Xiluan and Guo, Liangran and Gao, Xiaoling and Qiu, Mingfeng and Jiang, Xinguo and Lai, Ren and Chen, Hongzhuan
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2012): 368-78
Authors: Wu, Hongbing and Li, Jianxu and Zhang, Qizhi and Yan, Xiluan and Guo, Liangran and Gao, Xiaoling and Qiu, Mingfeng and Jiang, Xinguo and Lai, Ren and Chen, Hongzhuan
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2012): 368-78
A novel coumarin fluorescent sensor to probe polarity around biomolecules.
Authors: Signore, Giovanni and Nifosì, Riccardo and Albertazzi, Lorenzo and Bizzarri, Ranieri
Journal: Journal of biomedical nanotechnology (2009): 722-9
Authors: Signore, Giovanni and Nifosì, Riccardo and Albertazzi, Lorenzo and Bizzarri, Ranieri
Journal: Journal of biomedical nanotechnology (2009): 722-9
Directed immobilization of nucleic acids at ultramicroelectrodes using a novel electro-deposited polymer.
Authors: Gajovic-Eichelmann, Nenad and Ehrentreich-Förster, Eva and Bier, Frank F
Journal: Biosensors & bioelectronics (2003): 417-22
Authors: Gajovic-Eichelmann, Nenad and Ehrentreich-Förster, Eva and Bier, Frank F
Journal: Biosensors & bioelectronics (2003): 417-22
A scintillation proximity assay amenable for screening and characterization of DNA gyrase B subunit inhibitors.
Authors: Gevi, Monica and Domenici, Enrico
Journal: Analytical biochemistry (2002): 34-9
Authors: Gevi, Monica and Domenici, Enrico
Journal: Analytical biochemistry (2002): 34-9
Evaluation of biotin-dye conjugates for use in an HPLC assay to assess relative binding of biotin derivatives with avidin and streptavidin.
Authors: Wilbur, D S and Pathare, P M and Hamlin, D K and Frownfelter, M B and Kegley, B B and Leung, W Y and Gee, K R
Journal: Bioconjugate chemistry (2000): 584-98
Authors: Wilbur, D S and Pathare, P M and Hamlin, D K and Frownfelter, M B and Kegley, B B and Leung, W Y and Gee, K R
Journal: Bioconjugate chemistry (2000): 584-98