Name: mFluor™ UV455-streptavidin conjugate
Brand: AAT Bioquest
SKU: 47200
Availability: InStock

mFluor™ UV455-streptavidin conjugate

mFluor™ UV455 streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ UV 455 fluorescent dyes. Its high affinity for biotinylated conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ UV455 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by an ultraviolet laser, mFluor™ UV455 exhibits a bright and photostable emission of blue fluorescence at 461 nm. mFluor™ UV455 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.

Protocol

COA

Catalog	Size	Price	Quantity
47200	100 ug	Price
47201	1 mg	Price

References

View all 12 references: Citation Explorer

Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore.

Authors:

Lee, Molly M and Peterson, Blake R

Journal:

ACS omega (2016): 1266-1276

Fluorescence-based screening assays for the NAD⁺-dependent histone deacetylase smSirt2 from Schistosoma mansoni.

Authors:

Schiedel, Matthias and Marek, Martin and Lancelot, Julien and Karaman, Berin and Almlöf, Ingrid and Schultz, Johan and Sippl, Wolfgang and Pierce, Raymond J and Romier, Christophe and Jung, Manfred

Journal:

Journal of biomolecular screening (2015): 112-21

Discrimination between streptavidin and avidin with fluorescent affinity-based probes.

Authors:

Sun, Qian and Tian, Haiyu and Qu, Haoran and Sun, Deheng and Chen, Zhuo and Duan, Liping and Zhang, Weibing and Qian, Junhong

Journal:

The Analyst (2015): 4648-53

Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells.

Authors:

Sun, Qian and Qian, Junhong and Tian, Haiyu and Duan, Liping and Zhang, Weibing

Journal:

Chemical communications (Cambridge, England) (2014): 8518-21

Discovery of coumarin derivatives as fluorescence acceptors for intrinsic fluorescence resonance energy transfer of proteins.

Authors:

Kim, Ju Hwan and Sumranjit, Jitapa and Kang, Hyo Jin and Chung, Sang J

Journal:

Molecular bioSystems (2014): 30-3

Physical properties

Molecular weight	~52 kDa
Solvent	Water

Spectral properties

Absorbance (nm)	359
Correction factor (260 nm)	0.651
Correction factor (280 nm)	0.406
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	357
Emission (nm)	461
Quantum yield	0.42 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 16, 2025