logo
AAT Bioquest

mFluor™ Violet 500 Anti-human CD229 Antibody *HLy9.25*

The HLy9.25 monoclonal antibody reacts with human CD229, a 100 - 120 kD transmembrane protein frequently found on the surface of thymocytes, B cells, T cells and natural killer cells. In some organisms, CD229 positively regulates interleukin-17 production, and is associated with a variety of biologically interesting macromolecules/ligands, namely, SAP. CD229 is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD229 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 500 (ex/em = 410/501 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd229 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on August 23, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1229010012290101
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other namesLy9
CloneHLy9.25
Hostmouse
IsotypeMouse IgG1
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)

412

Correction Factor (260 nm)

0.769

Correction Factor (280 nm)

0.365

Extinction coefficient (cm -1 M -1)

250001

Excitation (nm)

410

Emission (nm)

501

Quantum yield

0.811

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Select Your CD Antibody
Select Your Clone
Select Your Conjugate