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mFluor™ Violet 500 Biotin Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 742.82 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 412 |
| Correction Factor (260 nm) | 0.769 |
| Correction Factor (280 nm) | 0.365 |
| Extinction coefficient (cm -1 M -1) | 250001 |
| Excitation (nm) | 410 |
| Emission (nm) | 501 |
| Quantum yield | 0.811 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: mFluor™ Dyes and Kits
Molecular weight 742.82 | Absorbance (nm) 412 | Correction Factor (260 nm) 0.769 | Correction Factor (280 nm) 0.365 | Extinction coefficient (cm -1 M -1) 250001 | Excitation (nm) 410 | Emission (nm) 501 | Quantum yield 0.811 |
mFluor™ Violet 500 Biotin Conjugate contains mFluor™ Violet 500 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to BD Horizon™ V500 and demonstrates excellent avidin binding and strong green fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 500 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 500 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 134.622 µL | 673.111 µL | 1.346 mL | 6.731 mL | 13.462 mL |
| 5 mM | 26.924 µL | 134.622 µL | 269.244 µL | 1.346 mL | 2.692 mL |
| 10 mM | 13.462 µL | 67.311 µL | 134.622 µL | 673.111 µL | 1.346 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 412 |
| Correction Factor (260 nm) | 0.769 |
| Correction Factor (280 nm) | 0.365 |
| Extinction coefficient (cm -1 M -1) | 250001 |
| Excitation (nm) | 410 |
| Emission (nm) | 501 |
| Quantum yield | 0.811 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 540 Biotin Conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
Images
Figure 1. mFluor™ Violet 500 Biotin Conjugate contains mFluor™ Violet 500 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to BD Horizon™ V500 and demonstrates excellent avidin binding and strong green fluorescence.
References
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Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
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Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
The poly-thymine based DNA photolithography onto electrostatic coupling substrates.
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
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Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.
Authors: Cheng, Na and Liu, Yujie and Mukama, Omar and Han, Xiaobo and Huang, Hualin and Li, Shuai and Zhou, Peng and Lu, Xuewen and Li, Zhiyuan
Journal: RSC advances (2020): 18601-18607
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Journal: RSC advances (2020): 18601-18607
Protein-induced fluorescence enhancement for a simple and universal detection of protein/small molecule interactions.
Authors: Kim, Hansol and Lee, Chang Yeol and Song, Jayeon and Yoon, Junhyeok and Park, Ki Soo and Park, Hyun Gyu
Journal: RSC advances (2018): 39913-39917
Authors: Kim, Hansol and Lee, Chang Yeol and Song, Jayeon and Yoon, Junhyeok and Park, Ki Soo and Park, Hyun Gyu
Journal: RSC advances (2018): 39913-39917
Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005
Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment.
Authors: Lei, Zhen and Chen, Hongda and Zhang, Hua and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
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