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mFluor™ Violet 500 Biotin Conjugate

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Physical properties
Molecular weight742.82
SolventDMSO
Spectral properties
Absorbance (nm)412
Correction Factor (260 nm)0.769
Correction Factor (280 nm)0.365
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)410
Emission (nm)501
Quantum yield0.811
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L)
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Show More (151)

OverviewpdfSDSpdfProtocol


Molecular weight
742.82
Absorbance (nm)
412
Correction Factor (260 nm)
0.769
Correction Factor (280 nm)
0.365
Extinction coefficient (cm -1 M -1)
250001
Excitation (nm)
410
Emission (nm)
501
Quantum yield
0.811
mFluor™ Violet 500 Biotin Conjugate contains mFluor™ Violet 500 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to BD Horizon™ V500 and demonstrates excellent avidin binding and strong green fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 500 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 500 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM134.622 µL673.111 µL1.346 mL6.731 mL13.462 mL
5 mM26.924 µL134.622 µL269.244 µL1.346 mL2.692 mL
10 mM13.462 µL67.311 µL134.622 µL673.111 µL1.346 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)412
Correction Factor (260 nm)0.769
Correction Factor (280 nm)0.365
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)410
Emission (nm)501
Quantum yield0.811

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 540 Biotin Conjugate4025351800010.2111.3260.543

Images


References


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Journal: The international journal of neuropsychopharmacology (2022): 328-338
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Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566
Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.
Authors: Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang
Journal: Chemical science (2021): 12407-12418
Global Detection of Proteins by Label-Based Antibody Array.
Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.
Authors: Cheng, Na and Liu, Yujie and Mukama, Omar and Han, Xiaobo and Huang, Hualin and Li, Shuai and Zhou, Peng and Lu, Xuewen and Li, Zhiyuan
Journal: RSC advances (2020): 18601-18607
The poly-thymine based DNA photolithography onto electrostatic coupling substrates.
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
Protein-induced fluorescence enhancement for a simple and universal detection of protein/small molecule interactions.
Authors: Kim, Hansol and Lee, Chang Yeol and Song, Jayeon and Yoon, Junhyeok and Park, Ki Soo and Park, Hyun Gyu
Journal: RSC advances (2018): 39913-39917
Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005
Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment.
Authors: Lei, Zhen and Chen, Hongda and Zhang, Hua and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: ACS applied materials & interfaces (2017): 44241-44250