AAT Bioquest

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

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Physical properties
Molecular weight256.15
Spectral properties
Correction Factor (280 nm)0.1
Extinction coefficient (cm -1 M -1)18000
Excitation (nm)360
Emission (nm)448
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM390.396 µL1.952 mL3.904 mL19.52 mL39.04 mL
5 mM78.079 µL390.396 µL780.793 µL3.904 mL7.808 mL
10 mM39.04 µL195.198 µL390.396 µL1.952 mL3.904 mL

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Spectral properties

Correction Factor (280 nm)0.1
Extinction coefficient (cm -1 M -1)18000
Excitation (nm)360
Emission (nm)448



View all 76 references: Citation Explorer
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Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
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Crystal structure of a covalent intermediate of endogenous human arylsulfatase A
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Journal: Food Chem Toxicol (2002): 1677
LVV-hemorphin-4 modulates Ca2+/calmodulin-dependent pathways in the immune system by the same mechanism as in the brain
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