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MycoLight™ Live Bacteria Fluorescence Imaging Kit
The MycoLight™ Live Bacteria Fluorescence Imaging Kit provides an easy and convenient way for visualizing live bacteria through fluorescent microscope. MycoLight™ 520 is non-fluorescent esterase substrate that diffuse into both Gram positive and Gram-negative bacteria. Upon hydrolysis by bacterial intracellular non-specific esterase, a green fluorescent product is produced and accumulated within bacteria. Compare to the commonly used esterase substrate CFDA and CFDA-AM, the kit provides brighter and more stable signal with lower background and easier staining protocol.
Fluorescence images of <em>E.coli</em> stained with CFDA or MycoLight&trade; Live Bacteria Fluorescence Imaging Kit. CFDA requires washing steps before imaging to minimize background, while no washing is needed using this kit (Cat#22409). The staining efficiency of MycoLight&trade; 520 is much higher than CFDA as more bacteria show green fluorescence. The signal of MycoLight&trade; 520 remains in cells after 1 hour of staining while CFDA leaks out readily. Same amount of bacteria were presented in each sample and fluorescence images were taken under the same exposure time.
Fluorescence images of <em>E.coli</em> stained with CFDA or MycoLight&trade; Live Bacteria Fluorescence Imaging Kit. CFDA requires washing steps before imaging to minimize background, while no washing is needed using this kit (Cat#22409). The staining efficiency of MycoLight&trade; 520 is much higher than CFDA as more bacteria show green fluorescence. The signal of MycoLight&trade; 520 remains in cells after 1 hour of staining while CFDA leaks out readily. Same amount of bacteria were presented in each sample and fluorescence images were taken under the same exposure time.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22409100 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.31
Correction factor (280 nm)0.12
Extinction coefficient (cm -1 M -1)
76000
Excitation (nm)498
Emission (nm)526
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation488 nm
Emission530 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 3, 2025