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Nuclear Red™ LCS1 *5 mM DMSO Solution*

Fluorescence images of HeLa cells stained with MitoLite™ Green FM using fluorescence microscope with a FITC filter set (Green). Live cells were co-stained with nuclei stain Nuclear Red™ LCS1 Cat. 17542 (Red).
Fluorescence images of HeLa cells stained with MitoLite™ Green FM using fluorescence microscope with a FITC filter set (Green). Live cells were co-stained with nuclei stain Nuclear Red™ LCS1 Cat. 17542 (Red).
Ordering information
Price ()
Catalog Number17542
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight627.27
Spectral properties
Excitation (nm)622
Emission (nm)645
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Nuclear Red™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Red™ LCS1 has its red fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Red™ LCS1 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.421 µL797.105 µL1.594 mL7.971 mL15.942 mL
5 mM31.884 µL159.421 µL318.842 µL1.594 mL3.188 mL
10 mM15.942 µL79.71 µL159.421 µL797.105 µL1.594 mL

Molarity calculator

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Spectral properties

Excitation (nm)622
Emission (nm)645

Product family

NameExcitation (nm)Emission (nm)
Nuclear Green™ LCS1 *5 mM DMSO Solution*503527
Nuclear Orange™ LCS1 *5 mM DMSO Solution*514556


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In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
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Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
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Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
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Journal: Anal Bioanal Chem (2008): 887
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
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Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
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Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
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