AAT Bioquest

OG488 BAPTA-1, AM [equivalent to Oregon Green® 488 BAPTA-1, AM] *Cell permeant*

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Physical properties
Dissociation constant (Kd, nM)170
Molecular weight1258.07
Spectral properties
Excitation (nm)493
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Dissociation constant (Kd, nM)
Excitation (nm)
Emission (nm)
OG488 BAPTA -1 AM is the same molecule of Oregon Green 488 BAPTA-1 AM ester. It is a cell-permeable and visible light-excitable calcium indicator that is often used with FITC filter set. Cells may be loaded with OG488 BAPTA -1 AM by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.


Flow cytometer

Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader

Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling

Example protocol


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

OG488 BAPTA-1 AM Stock Solution
  1. Prepare a 2 to 5 mM stock solution of OG488 BAPTA-1 AM in high-quality, anhydrous DMSO.


OG488 BAPTA-1 AM Working Solution
  1. On the day of the experiment, either dissolve OG488 BAPTA-1 AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature.

  2. Prepare a 2 to 20 µM OG488 BAPTA-1 AM working solution in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, OG488 BAPTA-1 AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.

    Note: The nonionic detergent Pluronic® F-127 is sometimes used to increase the aqueous solubility of OG488 BAPTA-1 AM. A variety of Pluronic® F-127 solutions can be purchased from AAT Bioquest.

    Note: If your cells contain organic anion-transporters, probenecid (1-2 mM) may be added to the dye working solution (final in well concentration will be 0.5-1 mM) to reduce leakage of the de-esterified indicators. A variety of ReadiUse™ Probenecid products, including water-soluble, sodium salt, and stabilized solutions, can be purchased from AAT Bioquest.


Following is our recommended protocol for loading AM esters into live cells. This protocol only provides a guideline and should be modified according to your specific needs.

  1. Prepare cells in growth medium overnight.
  2. On the next day, add 1X OG488 BAPTA-1 AM working solution to your cell plate.

    Note: If your compound(s) interfere with the serum, replace the growth medium with fresh HHBS buffer before dye-loading.

  3. Incubate the dye-loaded plate in a cell incubator at 37 °C for 30 to 60 minutes.

    Note: Incubating the dye for longer than 1 hour can improve signal intensities in certain cell lines.

  4. Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes.
  5. Add the stimulant as desired and simultaneously measure fluorescence using either a fluorescence microscope equipped with a FITC filter set or a fluorescence plate reader containing a programmable liquid handling system such as an FDSS, FLIPR, or FlexStation, at Ex/Em = 490/525 nm cutoff 515 nm.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of OG488 BAPTA-1, AM [equivalent to Oregon Green® 488 BAPTA-1, AM] *Cell permeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM79.487 µL397.434 µL794.868 µL3.974 mL7.949 mL
5 mM15.897 µL79.487 µL158.974 µL794.868 µL1.59 mL
10 mM7.949 µL39.743 µL79.487 µL397.434 µL794.868 µL

Molarity calculator

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Spectral properties

Excitation (nm)493
Emission (nm)522



View all 1 citations: Citation Explorer
Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain
Authors: Birkner, Antje
Journal: (2019)


View all 38 references: Citation Explorer
Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18
Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61
Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243
Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477
Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339
Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804
Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J., undefined
Journal: Am J Physiol Cell Physiol (2011): C266
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155
Voltage-gated calcium channels are involved in the regulation of calcium oscillations in vascular smooth muscle cells from isolated porcine retinal arterioles
Authors: Misfeldt MW, Aalkjaer C, Simonsen U, Bek T.
Journal: Exp Eye Res (2010): 69