OG488 BAPTA-1, AM [equivalent to Oregon Green® 488 BAPTA-1, AM] *Cell permeant*

OG488 BAPTA-1, AM

equivalent to Oregon Green® 488 BAPTA-1, AM; Cell permeant

OG488 BAPTA -1 AM is the same molecule of Oregon Green 488 BAPTA-1 AM ester. It is a cell-permeable and visible light-excitable calcium indicator that is often used with FITC filter set. Cells may be loaded with OG488 BAPTA -1 AM by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20507	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of OG488 BAPTA-1, AM [equivalent to Oregon Green® 488 BAPTA-1, AM] *Cell permeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	79.487 µL	397.434 µL	794.868 µL	3.974 mL	7.949 mL
5 mM	15.897 µL	79.487 µL	158.974 µL	794.868 µL	1.59 mL
10 mM	7.949 µL	39.743 µL	79.487 µL	397.434 µL	794.868 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1258.07 g/mol	=	mL	x	mM	=	mmol

References

View all 38 references: Citation Explorer

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla

Authors:

Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.

Journal:

Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures

Authors:

Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.

Journal:

Epilepsy Behav (2015): 61

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame

Authors:

Tomek J, Novak O, Syka J.

Journal:

J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons

Authors:

Schmitz J, Hoger U, Torkkeli PH, French AS.

Journal:

J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex

Authors:

Langer D, Helmchen F.

Journal:

Pflugers Arch (2012): 339

Physical properties

Dissociation constant (K_d, nM)	170
Molecular weight	1258.07
Solvent	DMSO

Spectral properties

Excitation (nm)	493
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on September 30, 2025