OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 170 |
Molecular weight | 1114.28 |
Solvent | Water |
Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Calcium Indicators, Cell Signaling Pathways
Molecular weight 1114.28 | Dissociation constant (Kd, nM) 170 | Excitation (nm) 493 | Emission (nm) 522 |
OG488 BAPTA -1 is the same molecule of Oregon Green 488 BAPTA-1. It is a visible light-excitable calcium indicator that is often used with FITC filter set. OG 488 BAPTA-1 has excitation and emission at 494 and 523 nm respectively. Its fluorescence intensity increases by ~14 fold upon binding calcium ion. This water-soluble, salt form is useful for intracellular loading by microinjection or infusion from patch pipette. The cell-permeant AM ester form of the indicator is also available (#20507).
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 89.744 µL | 448.72 µL | 897.44 µL | 4.487 mL | 8.974 mL |
5 mM | 17.949 µL | 89.744 µL | 179.488 µL | 897.44 µL | 1.795 mL |
10 mM | 8.974 µL | 44.872 µL | 89.744 µL | 448.72 µL | 897.44 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 38 references: Citation Explorer
Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18
Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61
Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243
Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477
Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339
Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804
Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J., undefined
Journal: Am J Physiol Cell Physiol (2011): C266
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J., undefined
Journal: Am J Physiol Cell Physiol (2011): C266
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155
Voltage-gated calcium channels are involved in the regulation of calcium oscillations in vascular smooth muscle cells from isolated porcine retinal arterioles
Authors: Misfeldt MW, Aalkjaer C, Simonsen U, Bek T.
Journal: Exp Eye Res (2010): 69
Authors: Misfeldt MW, Aalkjaer C, Simonsen U, Bek T.
Journal: Exp Eye Res (2010): 69
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?