Name: OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor
Brand: AAT Bioquest
SKU: 21511
Price: 308 USD
Availability: InStock

OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor

OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor is a mitochondria-targeted fluorescent probe that provides ratiometric detection of lipid peroxidation in live cells, offering high accuracy for complex biological systems.

Lipid Peroxidation Detection: Ratiometric shift from red to green upon oxidation
Mitochondria-Targeted: Lipophilic design ensures organelle-specific accumulation
Real-Time Monitoring: Enables tracking of oxidative stress dynamics
Quantitative Analysis: Ratio-based measurement corrects for dye loading variations

Fluorescence response of OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor (1000 nM) in HeLa cells with or without Erastin (10 µM) treatment at 37 ºC in a 5% CO2 incubator for 30 minutes. The fluorescence intensities were monitored with fluorescence microscopy using FITC and Cy3 filters.

Protocol

SDS

Catalog	Size	Price	Quantity
21511	5x100 ug	Price

Overview

A wide range of diseases is considered to result from mitochondrial oxidative damage that is associated with the lipid peroxidation of mitochondrial inner membranes. There are no specific methods to assess mitochondrial lipid peroxidation in live cells. To address this unmet need, AAT Bioquest has developed the OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor, a fluorescent mitochondria-targeted probe that detects lipid peroxidation in live cells. OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor enters cells rapidly, and selectively accumulates in mitochondria. It has a high specificity for the detection of mitochondrial lipid peroxidation. Mitochondrial lipid peroxidation results in a great change of fluorescence intensity ratio at 520 nm/590 nm, which can conveniently be monitored by fluorimetry, fluorescence microscopy or a fluorescence microplate reader. The fluorescence intensities are collected with FITC and Cy3/TRITC filter sets respectively at 520 nm/590 nm.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of OxiVision™ Ratiometric Mitochondrial Lipid Peroxidation Sensor to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	139.661 µL	698.305 µL	1.397 mL	6.983 mL	13.966 mL
5 mM	27.932 µL	139.661 µL	279.322 µL	1.397 mL	2.793 mL
10 mM	13.966 µL	69.83 µL	139.661 µL	698.305 µL	1.397 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	716.02 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

A Metastable Semiquinone Molecular Switch Modulated by Ascorbate/O2: A Study from a System Far-From-Equilibrium to Biological Assays in Mitochondria.

Authors:

Rodríguez-Valdez, Gabriela and Martínez-Cerda, Marlen E and Mejía-Reyes, Jisell G and Tapia-Juárez, Melissa and Olmos-Orizaba, Eridani and Cortés-Rojo, Christian and Cortés-García, Carlos J and Contreras-Celedón, Claudia A and Solorio-Alvarado, Cesar R and Chacón-García, Luis

Journal:

Chembiochem : a European journal of chemical biology (2024): e202400401

Synergy between nanoplastics and benzo[a]pyrene promotes senescence by aggravating ferroptosis and impairing mitochondria integrity in Caenorhabditis elegans.

Authors:

Ren, Huasheng and Yin, Kai and Lu, Xinhe and Liu, Jiaojiao and Li, Dandan and Liu, Zuojun and Zhou, Hailong and Xu, Shunqing and Li, Hanzeng

Journal:

The Science of the total environment (2024): 174418

Excitotoxicity, Oxytosis/Ferroptosis, and Neurodegeneration: Emerging Insights into Mitochondrial Mechanisms.

Authors:

Khan, Sameera and Bano, Nargis and Ahamad, Shakir and John, Urmilla and Dar, Nawab John and Bhat, Shahnawaz Ali

Journal:

Aging and disease (2024)

Ferroptosis Integrates Mitochondrial Derangements and Pathological Inflammation to Promote Pulmonary Hypertension.

Authors:

Kazmirczak, Felipe and Vogel, Neal T and Prisco, Sasha Z and Patterson, Michael T and Annis, Jeffrey and Moon, Ryan T and Hartweck, Lynn M and Mendelson, Jenna B and Kim, Minwoo and Mancipe, Natalia Calixto and Markowski, Todd and Higgins, LeAnn and Guerrero, Candace and Kremer, Ben and Blake, Madelyn L and Rhodes, Christopher J and Williams, Jesse W and Brittain, Evan L and Prins, Kurt W

Journal:

bioRxiv : the preprint server for biology (2024)

Cardiotoxicity of Iron and Zinc and Their Association with the Mitochondrial Unfolded Protein Response in Humans.

Authors:

Mirosevic, Vid and Svagusa, Tomo and Matic, Natalija and Maldini, Kresimir and Siljeg, Mario and Milicic, Davor and Gasparovic, Hrvoje and Rudez, Igor and Sepac, Ana and Gojmerac, Lucija and Kulic, Ana and Bakovic, Petra and Sedlic, Filip

Journal:

International journal of molecular sciences (2024)

Physical properties

Molecular weight	716.02
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC and Cy3/TRITC Filter
Emission	FITC and Cy3/TRITC Filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on May 5, 2026