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PE Anti-human CD3 Antibody
HIT3a
HIT3a is an anti-human monoclonal antibody that recognizes the CD3e antigen. CD3e (sometimes referred to as T cell antigen receptor complex or T3E) is a 20 kD member of the Ig superfamily that is found on the surface of cells like T cells. CD3 acts in essential cellular pathways, namely, the G protein-coupled receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. In addition, in certain organisms, it positively regulates T cell anergy, acts to positively regulate cell-matrix adhesion and is involved in the positive regulation of calcium-mediated signaling. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE (ex/em = 566/574 nm). It is compatible with the 561 nm laser and 585/16 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 100301L0 | 25 tests | Price | |
| 100301L1 | 100 tests | Price | |
| 100301L2 | 500 tests | Price |
Antibody properties
| Other names | T3E |
| Clone | HIT3a |
| Host | mouse |
| Isotype | mouse IgG2a |
| Reactivity | human |
Spectral properties
| Correction factor (280 nm) | 0.175 |
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 574 |
| Quantum yield | 0.82 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 3, 2025