Name: PE-iFluor® 660 Tandem
Brand: AAT Bioquest
SKU: 2602
Price: 232 USD
Availability: InStock

PE-iFluor® 660 Tandem

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.

Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2602	1 mg	Price

References

View all 35 references: Citation Explorer

Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.

Authors:

Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard

Journal:

Immunity (2021): 1578-1593.e5

Protocol for Creating Antibodies with Complex Fluorescence Spectra.

Authors:

McCarthy, Madeline E and Anglin, Caitlin M and Peer, Heather A and Boleman, Sevanna A and Klaubert, Stephanie R and Birtwistle, Marc R

Journal:

Bioconjugate chemistry (2021): 1156-1166

Methotrexate Hampers Immunogenicity to BNT162b2 mRNA COVID-19 Vaccine in Immune-Mediated Inflammatory Disease.

Authors:

Haberman, Rebecca H and Herati, Ramin Sedaghat and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya and Neurath, Markus and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U

Journal:

medRxiv : the preprint server for health sciences (2021)

Normal Numbers of Stem Cell Memory T Cells Despite Strongly Reduced Naive T Cells Support Intact Memory T Cell Compartment in Ataxia Telangiectasia.

Authors:

Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam

Journal:

Frontiers in immunology (2021): 686333

Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells.

Authors:

Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo

Journal:

American journal of respiratory and critical care medicine (2021): 1230-1244

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	695

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025