PE-iFluor® 660 Tandem
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 566 |
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 695 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS), PE and APC, Spectral Flow Cytometry
Absorbance (nm) 566 | Extinction coefficient (cm -1 M -1) 1960000 | Excitation (nm) 565 | Emission (nm) 695 |
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 566 |
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 695 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
| PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
| PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
| PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
| PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
| PE-iFluor® 594 Tandem | 565 | 606 | 1960000 |
| PE-iFluor™ 597 Tandem | 565 | 612 | - |
| PE-iFluor™ 740 Tandem | 565 | 767 | 1960000 |
| PE-iFluor™ 720 Tandem | 565 | 750 | 1960000 |
Show More (1) | |||
Images
Figure 1. Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 660 specific B9-A channel.
Figure 2. The fluorescence intensity of PE-iFluor® 660 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.
References
View all 35 references: Citation Explorer
Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5
Use of Toll-Like Receptor (TLR) Ligation to Characterize Human Regulatory B-Cells Subsets.
Authors: Chayé, Mathilde A M and Tontini, Chiara and Ozir-Fazalalikhan, Arifa and Voskamp, Astrid L and Smits, Hermelijn H
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 235-261
Authors: Chayé, Mathilde A M and Tontini, Chiara and Ozir-Fazalalikhan, Arifa and Voskamp, Astrid L and Smits, Hermelijn H
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 235-261
Distinct cellular immune profiles in the airways and blood of critically ill patients with COVID-19.
Authors: Saris, Anno and Reijnders, Tom Dy and Nossent, Esther J and Schuurman, Alex R and Verhoeff, Jan and Asten, Saskia van and Bontkes, Hetty and Blok, Siebe and Duitman, Janwillem and Bogaard, Harm-Jan and Heunks, Leo and Lutter, Rene and van der Poll, Tom and Garcia Vallejo, Juan J and ,
Journal: Thorax (2021)
Authors: Saris, Anno and Reijnders, Tom Dy and Nossent, Esther J and Schuurman, Alex R and Verhoeff, Jan and Asten, Saskia van and Bontkes, Hetty and Blok, Siebe and Duitman, Janwillem and Bogaard, Harm-Jan and Heunks, Leo and Lutter, Rene and van der Poll, Tom and Garcia Vallejo, Juan J and ,
Journal: Thorax (2021)
Methotrexate hampers immunogenicity to BNT162b2 mRNA COVID-19 vaccine in immune-mediated inflammatory disease.
Authors: Haberman, Rebecca H and Herati, Ramin and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya M and Neurath, Markus F and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: Annals of the rheumatic diseases (2021)
Authors: Haberman, Rebecca H and Herati, Ramin and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya M and Neurath, Markus F and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: Annals of the rheumatic diseases (2021)
Basophils promote barrier dysfunction and resolution in the atopic skin.
Authors: Pellefigues, Christophe and Naidoo, Karmella and Mehta, Palak and Schmidt, Alfonso J and Jagot, Ferdinand and Roussel, Elsa and Cait, Alissa and Yumnam, Bibek and Chappell, Sally and Meijlink, Kimberley and Camberis, Mali and Jiang, Jean X and Painter, Gavin and Filbey, Kara and Uluçkan, Özge and Gasser, Olivier and Le Gros, Graham
Journal: The Journal of allergy and clinical immunology (2021)
Authors: Pellefigues, Christophe and Naidoo, Karmella and Mehta, Palak and Schmidt, Alfonso J and Jagot, Ferdinand and Roussel, Elsa and Cait, Alissa and Yumnam, Bibek and Chappell, Sally and Meijlink, Kimberley and Camberis, Mali and Jiang, Jean X and Painter, Gavin and Filbey, Kara and Uluçkan, Özge and Gasser, Olivier and Le Gros, Graham
Journal: The Journal of allergy and clinical immunology (2021)
IFNγ-producing CD8+ tissue resident memory T cells are a targetable hallmark of immune checkpoint inhibitor-colitis.
Authors: Sasson, Sarah C and Slevin, Stephanie M and Cheung, Vincent Tf and Nassiri, Isar and Olsson-Brown, Anna and Fryer, Eve and Ferreira, Ricardo C and Trzupek, Dominik and Gupta, Tarun and Al-Hillawi, Lulia and Issaias, Mari-Lenna and Easton, Alistair and Campo, Leticia and FitzPatrick, Michael Eb and Adams, Joss and Chitnis, Meenali and Protheroe, Andrew and Tuthill, Mark and Coupe, Nicholas and Simmons, Alison and Payne, Miranda and Middleton, Mark R and Travis, Simon Pl and , and Fairfax, Benjamin P and Klenerman, Paul and Brain, Oliver
Journal: Gastroenterology (2021)
Authors: Sasson, Sarah C and Slevin, Stephanie M and Cheung, Vincent Tf and Nassiri, Isar and Olsson-Brown, Anna and Fryer, Eve and Ferreira, Ricardo C and Trzupek, Dominik and Gupta, Tarun and Al-Hillawi, Lulia and Issaias, Mari-Lenna and Easton, Alistair and Campo, Leticia and FitzPatrick, Michael Eb and Adams, Joss and Chitnis, Meenali and Protheroe, Andrew and Tuthill, Mark and Coupe, Nicholas and Simmons, Alison and Payne, Miranda and Middleton, Mark R and Travis, Simon Pl and , and Fairfax, Benjamin P and Klenerman, Paul and Brain, Oliver
Journal: Gastroenterology (2021)
Multibatch Cytometry Data Integration for Optimal Immunophenotyping.
Authors: Ogishi, Masato and Yang, Rui and Gruber, Conor and Zhang, Peng and Pelham, Simon J and Spaan, András N and Rosain, Jérémie and Chbihi, Marwa and Han, Ji Eun and Rao, V Koneti and Kainulainen, Leena and Bustamante, Jacinta and Boisson, Bertrand and Bogunovic, Dusan and Boisson-Dupuis, Stéphanie and Casanova, Jean-Laurent
Journal: Journal of immunology (Baltimore, Md. : 1950) (2021): 206-213
Authors: Ogishi, Masato and Yang, Rui and Gruber, Conor and Zhang, Peng and Pelham, Simon J and Spaan, András N and Rosain, Jérémie and Chbihi, Marwa and Han, Ji Eun and Rao, V Koneti and Kainulainen, Leena and Bustamante, Jacinta and Boisson, Bertrand and Bogunovic, Dusan and Boisson-Dupuis, Stéphanie and Casanova, Jean-Laurent
Journal: Journal of immunology (Baltimore, Md. : 1950) (2021): 206-213
Detecting Förster resonance energy transfer in living cells by conventional and spectral flow cytometry.
Authors: Henderson, Jared and Havranek, Ondrej and Ma, Man Chun John and Herman, Vaclav and Kupcova, Kristyna and Chrbolkova, Tereza and Pacheco-Blanco, Mariana and Wang, Zhiqiang and Comer, Justin M and Zal, Tomasz and Davis, Richard Eric
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Authors: Henderson, Jared and Havranek, Ondrej and Ma, Man Chun John and Herman, Vaclav and Kupcova, Kristyna and Chrbolkova, Tereza and Pacheco-Blanco, Mariana and Wang, Zhiqiang and Comer, Justin M and Zal, Tomasz and Davis, Richard Eric
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells.
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244
Normal Numbers of Stem Cell Memory T Cells Despite Strongly Reduced Naive T Cells Support Intact Memory T Cell Compartment in Ataxia Telangiectasia.
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333