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AAT Bioquest

PE/Texas Red® Anti-human CD73 Antibody *AD2*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)613
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
NT5E; Ecto-5'-nucleotidase
Clone
AD2
Host
mouse
Isotype
Mouse IgG1 kappa
Reactivity
human
Application
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)
565
Emission (nm)
613
AD2 is an anti-human monoclonal antibody that is specific for the CD73 antigen. CD73 (sometimes called NT5E or Ecto-5'-nucleotidase) is a 70 kD transmembrane protein that is found on the surface of cells such as epithelial cells, endothelial cells and stem cells. CD73 is associated with a variety of biologically interesting macromolecules/ligands, for instance, AMP. CD73 is a moderately popular antibody target, with over 11000 publications in the last decade. CD73 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells. This antibody was purified through affinity chromatography and conjugated to PE/Texas Red® (ex/em = 567/615 nm). It is compatible with the 561 nm laser and 610/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)613

Antibody Finder


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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd73 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.