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PerCP-iFluor® 710

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). PerCP-iFluor® 710 tandem conjugates can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP-iFluor® 710 conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PerCP-iFluor® 700675707350000
PerCP-iFluor® 780778806350000
iFluor® 710 amine7167391500001

References

View all 13 references: Citation Explorer
Stark fluorescence spectroscopy on peridinin-chlorophyll-protein complex of dinoflagellate, Amphidinium carterae.
Authors: Ara, Anjue Mane and Shakil Bin Kashem, Md and van Grondelle, Rienk and Wahadoszamen, Md
Journal: Photosynthesis research (2020): 233-239
Comparative Analysis of Bone Marrow-derived Mast Cell Differentiation in C57BL/6 and BALB/c Mice.
Authors: Nagashima, Miki and Koyanagi, Madoka and Arimura, Yutaka
Journal: Immunological investigations (2019): 303-320
Exogenous IL-9 Ameliorates Experimental Autoimmune Myasthenia Gravis Symptoms in Rats.
Authors: Yao, Xiuhua and Zhao, Jiarui and Kong, Qingfei and Xie, Xiaoli and Wang, Jinghua and Sun, Bo and Xu, Lixia and Mu, Lili and Li, Hulun
Journal: Immunological investigations (2018): 712-724
Triplet-triplet energy transfer from chlorophylls to carotenoids in two antenna complexes from dinoflagellate Amphidinium carterae.
Authors: Kvíčalová, Zuzana and Alster, Jan and Hofmann, Eckhard and Khoroshyy, Petro and Litvín, Radek and Bína, David and Polívka, Tomáš and Pšenčík, Jakub
Journal: Biochimica et biophysica acta (2016): 341-9
Effects of the fusion design and immunization route on the immunogenicity of Ag85A-Mtb32 in adenoviral vectored tuberculosis vaccine.
Authors: Zhang, Yiling and Feng, Liqiang and Li, Liang and Wang, Dimin and Li, Chufang and Sun, Caijun and Li, Pingchao and Zheng, Xuehua and Liu, Yichu and Yang, Wei and Niu, Xuefeng and Zhong, Nanshan and Chen, Ling
Journal: Human vaccines & immunotherapeutics (2015): 1803-13
Page updated on December 13, 2024

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Catalog Number2652
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

350000

Excitation (nm)

719

Emission (nm)

747

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
Flow cytometry analysis of PBMC stained with PerCP-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 710 specific B12-A channel.
Flow cytometry analysis of PBMC stained with PerCP-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 710 specific B12-A channel.
Flow cytometry analysis of PBMC stained with PerCP-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 710 specific B12-A channel.