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PerCP-iFluor® 750
Product key features
- Excitation/Emission Maxima: Efficient excitation at 488 nm with peak emission near 784 nm, enabling detection in the near-infrared region.
- Low Spectral Overlap: Minimal spillover into adjacent channels facilitates cleaner separation in high-parameter flow cytometry panels.
- High Signal Intensity: Delivers bright far-red fluorescence with excellent sensitivity and low background.
- Large Stokes Shift: Enhances spectral resolution by maximizing the separation between excitation and emission wavelengths.
Product description
PerCP-iFluor® 750 is a tandem fluorophore engineered for high-parameter flow cytometry, providing strong far-red fluorescence with minimal spectral spillover. It comprises a peridinin chlorophyll protein (PerCP) donor covalently linked to an iFluor® 750 acceptor, allowing efficient energy transfer through Förster resonance energy transfer (FRET). Upon excitation with a 488 nm blue laser, the conjugate emits maximally near 784 nm, yielding a large Stokes shift and enabling sensitive detection in the near-infrared region. Its distinct spectral characteristics make PerCP-iFluor® 750 highly suitable for use in complex multicolor panels, where it helps minimize compensation and improves resolution of closely spaced populations. The dye is ideal for applications such as immunophenotyping, rare cell analysis, and other advanced flow cytometry workflows requiring high signal-to-noise ratios and precise population discrimination. PerCP-iFluor® 750 can be conjugated to proteins and antibodies using SMCC chemistry or through site-specific conjugation with Buccutite™ MTA and Buccutite™ FOL.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PerCP-iFluor® 700 | 675 | 707 | 350000 |
| PerCP-iFluor® 710 | 719 | 747 | 350000 |
| PerCP-iFluor® 720 | 482 | 747 | - |
| PerCP-iFluor® 780 | 778 | 806 | 350000 |
References
View all 16 references: Citation Explorer
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Comparison of minimal residual disease detection in multiple myeloma by SRL 8-color single-tube and EuroFlow 8-color 2-tube multiparameter flow cytometry.
Authors: Takamatsu, Hiroyuki and Yoroidaka, Takeshi and Fujisawa, Momoko and Kobori, Kazuya and Hanawa, Masako and Yamashita, Takeshi and Murata, Ryoichi and Ueda, Mikio and Nakao, Shinji and Matsue, Kosei
Journal: International journal of hematology (2019): 377-381
Authors: Takamatsu, Hiroyuki and Yoroidaka, Takeshi and Fujisawa, Momoko and Kobori, Kazuya and Hanawa, Masako and Yamashita, Takeshi and Murata, Ryoichi and Ueda, Mikio and Nakao, Shinji and Matsue, Kosei
Journal: International journal of hematology (2019): 377-381
[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
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