Name: PhosLite™ Green
Brand: AAT Bioquest
SKU: 11630
Price: 232 USD
Availability: InStock

PhosLite™ Green

This water-soluble PhosLite™ Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique properties (such as an extremely large Stokes shift and high photostability), making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11630	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of PhosLite™ Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	169.638 µL	848.191 µL	1.696 mL	8.482 mL	16.964 mL
5 mM	33.928 µL	169.638 µL	339.276 µL	1.696 mL	3.393 mL
10 mM	16.964 µL	84.819 µL	169.638 µL	848.191 µL	1.696 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	589.49 g/mol	=	mL	x	mM	=	mmol

References

View all 31 references: Citation Explorer

The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake

Authors:

Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P.

Journal:

Plant Cell Environ (2012): 126

A novel fluorescent transcriptional reporter for cell-based microarray assays

Authors:

Redmond TM, Uhler MD.

Journal:

Methods Mol Biol (2011): 41

Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling

Authors:

Duhamel S, Gregori G, Van Wambeke F, Nedoma J.

Journal:

Cytometry A (2009): 163

Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays

Authors:

Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.

Journal:

J Neural Eng (2008): 203

Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats

Authors:

Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.

Journal:

Am J Physiol Gastrointest Liver Physiol (2007): G1223

Physical properties

Molecular weight	589.49
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 9, 2025