PhosLite™ Green

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	589.49
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

589.49

This water-soluble PhosLite™ Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique properties (such as an extremely large Stokes shift and high photostability), making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of PhosLite™ Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	169.638 µL	848.191 µL	1.696 mL	8.482 mL	16.964 mL
5 mM	33.928 µL	169.638 µL	339.276 µL	1.696 mL	3.393 mL
10 mM	16.964 µL	84.819 µL	169.638 µL	848.191 µL	1.696 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)
Indocyanine Green CAS 3599-32-4	789	813
Helixyte™ Green 20X Aqueous PCR Solution	498	522
Helixyte™ Green 10,000X Aqueous PCR Solution	498	522
Thiolite™ Green	505	524
CytoTell™ Green	510	525
LysoBrite™ Green	501	510
Q4ever™ Green 1250X DMSO Solution	503	527
Droplite™ Green	421	521

Images

Figure 1. Chemical structure for PhosLite™ Green

References

View all 31 references: Citation Explorer

The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake
Authors: Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P.
Journal: Plant Cell Environ (2012): 126

A novel fluorescent transcriptional reporter for cell-based microarray assays
Authors: Redmond TM, Uhler MD.
Journal: Methods Mol Biol (2011): 41

Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling
Authors: Duhamel S, Gregori G, Van Wambeke F, Nedoma J.
Journal: Cytometry A (2009): 163

Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays
Authors: Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.
Journal: J Neural Eng (2008): 203

Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Authors: Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.
Journal: Am J Physiol Gastrointest Liver Physiol (2007): G1223

Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation
Authors: Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T.
Journal: J Bone Miner Res (2006): 1012

Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry
Authors: Dignum M, Hoogveld HL, Matthijs HC, Laanbroek HJ, Pel R.
Journal: FEMS Microbiol Ecol (2004): 29

Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation
Authors: Basu S, Campagnola PJ.
Journal: Biomacromolecules (2004): 572

Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics
Authors: Nedoma J, Strojsova A, Vrba J, Komarkova J, Simek K.
Journal: Environ Microbiol (2003): 462

Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode
Authors: Telford WG, Hawley TS, Hawley RG.
Journal: Cytometry A (2003): 48