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PhosLite™ Green

This water-soluble PhosLite™ Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique properties (such as an extremely large Stokes shift and high photostability), making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of PhosLite™ Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM169.638 µL848.191 µL1.696 mL8.482 mL16.964 mL
5 mM33.928 µL169.638 µL339.276 µL1.696 mL3.393 mL
10 mM16.964 µL84.819 µL169.638 µL848.191 µL1.696 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References

View all 31 references: Citation Explorer
The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake
Authors: Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P.
Journal: Plant Cell Environ (2012): 126
A novel fluorescent transcriptional reporter for cell-based microarray assays
Authors: Redmond TM, Uhler MD.
Journal: Methods Mol Biol (2011): 41
Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling
Authors: Duhamel S, Gregori G, Van Wambeke F, Nedoma J.
Journal: Cytometry A (2009): 163
Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays
Authors: Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.
Journal: J Neural Eng (2008): 203
Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Authors: Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.
Journal: Am J Physiol Gastrointest Liver Physiol (2007): G1223
Page updated on December 6, 2024

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Catalog Number11630
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Physical properties

Molecular weight

589.49

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501