PhosLite™ Green

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Chemical structure for PhosLite™ Green
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Unit Size: Cat No: Price (USD): Qty:
1 mg 11630 $195

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Telephone: 1-800-990-8053
Fax: 1-408-733-1304
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Ex/Em (nm)345/520
Storage Freeze (<-15 °C)
Minimize light exposure
Category Enzyme Detection
Related Protein Phosphatase
This water-soluble PhosLite™ Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique properties (such as an extremely large Stokes shift and high photostability), making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of PhosLite™ Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =

References & Citations

The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake
Authors: Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P.
Journal: Plant Cell Environ (2012): 126

A novel fluorescent transcriptional reporter for cell-based microarray assays
Authors: Redmond TM, Uhler MD.
Journal: Methods Mol Biol (2011): 41

Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling
Authors: Duhamel S, Gregori G, Van Wambeke F, Nedoma J.
Journal: Cytometry A (2009): 163

Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays
Authors: Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.
Journal: J Neural Eng (2008): 203

Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Authors: Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.
Journal: Am J Physiol Gastrointest Liver Physiol (2007): G1223

Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation
Authors: Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T.
Journal: J Bone Miner Res (2006): 1012

Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry
Authors: Dignum M, Hoogveld HL, Matthijs HC, Laanbroek HJ, Pel R.
Journal: FEMS Microbiol Ecol (2004): 29

Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation
Authors: Basu S, Campagnola PJ.
Journal: Biomacromolecules (2004): 572

Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode
Authors: Telford WG, Hawley TS, Hawley RG.
Journal: Cytometry A (2003): 48

Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics
Authors: Nedoma J, Strojsova A, Vrba J, Komarkova J, Simek K.
Journal: Environ Microbiol (2003): 462

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Additional Documents

Safety Data Sheet (SDS)

1. Enzyme Probes & Assay Kits

Certificate of Analysis